EFFECTS OF THROMBOLYSIS WITH ULTRASOUND ON THE STRUCTURE OF ERYTHROCYTE AND ITS SAFETY THRESHOLD

The study was to investigate thrombolysis in vitro with ultrasound, and to discuss effects of thrombolysis with ultrasound on the structure of erythrocyte and its safety threshold under different ultrasound intensity and exposure time. The structure of erythrocyte in thrombus was evaluated under light microscope. The relationship between the structure of erythrocyte in thrombus and ultrasound intensity and exposure time was obtained. The results showed that ultrasound eliminated the thrombus. According to the change of the structure of erythrocyte in thrombus and ultrasound intensity and exposure time, the effects of thrombolysis with ultrasound could be divided into three kinds of areas： the A, B, C area. The area A was the safe area, the area B was the relatively safe area, and the area C was the irreversible damage area. The study suggested that ultrasound intensity and exposure time had significant impact on the structure of erythrocyte. Stronger ultrasound intensity or longer exposure time could cause erythrocytes irreversible damage. It could accelerate thrombolysis and shorten the exposure time that the ultrasound intensity was little bit increased. The study of effects of thrombolysis with ultrasound on the structure of erythrocyte and its safety threshold were important for practical applications.     

REAL-TIME DETECTION OF SURVIVIN mRNA EXPRESSION IN CERVICAL CANCER CELL LINES USING MOLECULAR BEACON IMAGING

Objective To detect the expression of survivin mRNA in cervical cancer cell lines using molecular beacon imaging technology. Methods Human cervical cancer cells （HeLa and SiHa） and human fetal lung fibroblast HFL-I were cultured in vitro. After adding 100 nmol/L survivin mRNA molecular beacon, the fluorescent signals were observed under fluorescent microscope. The expressions of survivin in cervical cancer cells and HFL-I cell were examined by immunocytochemical streptravidin-biothin peroxidase （SP） assay at the same time. Results Two kinds of survivin mRNA molecular beacon, with different color fluorescence, had strong fluorescent signal in cervical cancer cell lines, and the signal in SiHa cell line was stronger, but these signals were not found in HFL-I ; Immunocytochemical staining of positive survivin was located in the cytoplasm of cervical cancer cell lines HeLa and SiHa, whereas, no expression of survivin was detected in HFL-I cell line. Conclusion The technology of molecular beacon imaging can be used to detect the expression of survivin mRNA in viable cells successfully, and may provide a new approach to the diagnosis of early stage cervical cancer and the following-up in the clinic.     

EXPRESSION OF IL-2 AND SIL-2R AND ALTERATION OF CELL IMMUNITY IN PATIENTS WITH HYPERTENSIVE CEREBRAL HEMORRHAGE

Objective To study the expression of interleukin-2 （IL-2）, soluble interleukin-2 receptor （sIL-2R）, determine the alteration of erythrocytic immunity and T cell subgroup in the blood of outer circulation in patients with hypertensive cerebral hemorrhage so and to probe into the relationship between them, and to explore the clinical significance. Methods Enzyme linked immnunosorbent assay （ELISA） was used to determine the content of IL-2 and slL-2R. The immunoadsorption was employed to examine the erythrocytic immune activity and its regulating function. （ S-P） was used to determine the cell number of CD3 （cluster of differentiation3）, CD4 and CD8. Resolts The content of IL-2 in the group with hypertensive cerebral hemorrhage was significantly lower than that in the control group （P〈0.01）, and the content of sIL-2R increased. Red blood cell C3b receptor （RBC. C3bR） and RBC immune adherence enhancing factor （RFEB） dropped greatly （P〈0.01）, while RBC immune complex rosette （RBC. ICR） and RBC immune adherence inhibiting factor （RFIR） increased greatly. The cell number of CD3 and CD4decreased （P〈0.01） and there was no obvious change in CD8 （P〈 0. 05）. Conclusion The decrease of immune function was observed in patients with hypertensive cerebral hemorrhage. The determination of the content of IL-2, sIL- 2R, erythrocytic immunity and the activity of T subgroup has an important clinical significance in the occurrence, development, treatment, and prognosis of hypertensive cerebral hemorrhage.     

Economic viability of mega-size containership in different service networks

The mega-size containership viability was analyzed by considering different service networks for different ship sizes： hub-and-spoke and multi-port-calling （MPC） networks for mega-size containerships and conventional ships. A model was proposed, which quantifies the economies of scale in operating large containerships and constructs models for ship routing under different service networks. A sensitivity analysis was conducted to test the effect of feeder costs and the results analyzed to determine optimal containership size with respect to different operational scenarios. Throughout model applications for Asia-Europe and Asia-North America trades, the mega-size containership is competitive in all scenarios for Asia-Europe, while it is viable for Asia-North America only when the feeder costs are low.     

Binary Image Watermarking Algorithm Using Matrix of Complexity Index

A new watermarking algorithm of binary image is proposed.The complexity index of pixels is presented to reflect the change degree of pixels and to evaluate the modifiable degree of pixels.Firstly, in a small image block, the complexity index of"jumping-change"is calculated in vertical and horizontal direction.Secondly, the matrix of the complexity index is calculated by integrating the complexity index of pixels in two directions.Finally, the matrix of the complexity index is used to embed the watermark in binary images.Experimental results show that the proposed algorithm has a good performance.     

A Novel Model of F0 Contours Prediction for Continuous Speech

F0 （fundamental frequency） contour was studied under different prosodic environment in continuous speech and a novel model of F0 contours prediction was proposed. It describes syllabic F0 contour with two points, one curve and duration. The curve represents two optimal points of controlling parameters. The duration represents the syllabic duration. The prosodic characters of controlling parameters were analyzed by CART （Class and Regression Tree）. A set of controlling parameters was analyzed, which reflects the linguistic environment and prosodic structure. Then it sets up the model of F0 contours prediction with the two optimal controlling parameters and F0 templates. The end pitch value of previous syllable as special prosodic parameters was used to keep the continuity of fore-and-aft syllable. It focuses on looking out the main prosodic clues hiding in F0 contours and applying it to simplify the model for prediction. The results of synthesis experiment show that the performance of the prediction method is appreciated.     

Formalization of a New Approach Fault Analysis and of Fault Detection

Substitution permutation network （SPN） is one important structure of block cipher cryptosystems. Pri or work has shown different fault analyses on SPN. The formalization of fault analysis of both attack and protect on SPN have been given. The overhead and time tolerance of fault detection have been discussed. The pseudoblinding method to detect fault attack is introduced, and the balance of the security, overhead and time tolerance based on the evaluation could be made.     

Segmentation of haustral folds and polyps on haustral folds in CT colonography using complementary geodesic distance transformation

Computer-aided detection （CAD） for CT colonography refers to a scheme that automatically detects polyps in CT images of colon. Current CAD schemes already have a relatively high sensitivity and a low false positive rate. However, misdiagnosis and missed diagnosis are still common to happen, mainly due to the existence of haustral folds （HFs）. An innovative idea of segmenting semilunar HFs from the smooth colonic wall and then using different methods to detect polyps on HFs and those on the smooth colonic wall is proposed in this paper to reduce the false positives and false negatives caused by HFs. For the polyps on HFs, a novel segmentation method is specially developed based on complementary geodesic distance transformation （CGDT）. The proposed method is tested on four different models and real CT data, The property of CGDT is proved and our method turns out to be effective for HF segmentation and polyp segmentation. The encouraging experimental results primarily show the feasibility of the proposed method and its potential to improve the detection performance of CAD schemes.     

Experimental Investigation of the Influence of Fuel Viscosity on the Spray Characteristics of Diesel Nozzle

This paper presented an investigation of atomization characteristics including the velocity vector field and the mean droplet sizes for different percentages of DMM-diesel blended fuels using a phase doppler anemometry (PDA) analyzer system. Based on the fuel design concept, an oxygenated fuel named dimethoxy methane (DMM), which has lower viscosity, surface tension, and boiling point, was used to blend with diesel. The experiments were carried out under atmospheric conditions on a single-hole type diesel nozzle, liquid conditions comprise a temperature of 298 K under the needle valve opening pressure of 6 MPa. The results show that the sauter mean diameter (SMD) and spray cone angle of blended fuels decrease with the increase of DMM content; the axial mean velocity in the centerline increases with the increase of DMM. However, the spray behavior of blended fuel in which DMM exceeds 75% is virtually identical to that of neat DMM. The measurement also reveals the existence of an "S" shape in the radial mean velocity variations with radial distance.     

Radar Emitter Signal Recognition Based on Complexity Features

Intra-pulse characteristics of different radar emitter signals reflect on signal waveform by way of changing frequency, phase and amplitude. A novel approach was proposed to extract complexity features of radar emitter signals in a wide range of signal-to-noise ratio ( SNR), and radial basis probability neural network (RBPNN) was used to recognize different radar emitter signals. Complexity features, including Lempel-Ziv complexity (LZC) and correlation dimension (CD), can measure the complexity and irregularity of signals, which mirrors the intra-pulse modulation laws of radar emitter signals. In an experiment, LZC and CD features of 10 typical radar emitter signals were extracted and RBPNN was applied to identify the 10 radar emitter signals. Simulation results show that the proposed approach is effective and has good application values because average accurate recognition rate is high when SNR varies in a wide range.     

二氧化碳人工气腹在安氟醚吸入麻醉中对心率变异性及脑电指标的影响

目的　探讨CO2 人工气腹在安氟醚吸入麻醉中对腹腔镜胆囊切除手术患者心率变异性 (heartratevariability ,HRV)及脑电 (electroencephalogram ,EEG)指标的不同影响。 方法　利用心率变异功率谱分析技术 (HRV PSA)和脑电功率谱分析技术 (EEG PSA)对 2 8例腹腔镜胆囊切除手术患者在麻醉前、麻醉诱导插管后及CO2 人工气腹后HRV指标LF、HF、LF HF、TP及EEG参数BIS、95 %SEF的变化进行了观察。结果　麻醉诱导插管后LF、HF、TP、BIS及95 %SEF均显著降低 ,LF HF未见明显改变 ;CO2 人工气腹后LF、LF HF、TP显著升高 ,HF、BIS及 95 %SEF未见明显改变。结论　CO2 人工气腹使交感神经活性显著升高而对大脑皮层意识活动影响不大     

多发性腔隙性脑梗塞的EEG、BEAM、CT/MRI对比分析

对54例经颅脑CT(X线电予计算机断层扫描)或MRI(核磁共振成象)检查确诊为多发性腔隙性脑梗塞病人的EEG(脑电图)、BEAM(脑电地形图)检查结果进行了对比分析。结果表明,BEAM在定位价值上逊于CT或MRI,优于EEG(P<0.01),反映脑功能变化的敏感性方面,BEAM也优于EEG(P<0.05)。BEAM的改变以慢波频段能量增高明显,其分布部位以额颠部最明显,枕颠及额区次之(P<0.05)。认为BEAM对于多发性腔隙性脑梗塞病人不失为一种敏感性较高、对病人无损伤、价廉、易普及的早期辅助检查方法。     

应用气相色谱法测定静脉全身麻醉中病人呼吸气中二氧化碳浓度

本文报告了应用气相色谱测定静脉全身麻醉中病人呼吸气中CO_2气体浓度的一种方法。经应用证明方法简便,经济。这对了解病人通气功能加强呼吸管理起到一定指导作用。对一机多用有一定的启示。     

用于无线通信系统的信号强度检测电路设计

为了解决传统的信号检测电路准确度不高,对温度和工艺变化抵抗能力不够的问题,建立了信号强度检测电路的系统模型.推导得到限幅放大器增益与信号强度检测单元误差的关系以及降低信号强度检测单元误差的方法.通过分析,得到在提高准确度的前提下减小限幅放大器增益随温度和工艺变化的方法.基于以上分析,引入增益加强结构,以提高限幅放大器增益的准确性和抵抗温度及工艺变化的能力.采用0.18μm体硅(CMOS)工艺,1.8 V供电电源,面积为500μm×200μm的电路进行测试,结果表明:测试温度从-40°C变化到85°C时,该信号强度检测电路都可以提供55 dB的动态范围和大于55 MHz的带宽,其检测误差小于1.5 dB,电路功耗为1.89 mW,检测精度高达1.5 dB.     

有限快拍下的小尺度矢量阵DOA算法

针对小尺度矢量水听器阵条件下ESPRIT算法目标方位估计精度低、多目标分辨能力弱的缺点,提出了一种基于酉变换的DOA改进算法.该方法无需计算观测数据的协方差矩阵,利用阵列的观测数据及共轭信息构造一中心Hermitian对称矩阵,根据酉变换原理将其转化为实数矩阵并用实值ESPRIT方法估计目标方位.由于利用了观测数据的共轭信息,相当于虚拟增加了阵元个数,改进算法有更高的DOA估计精度和多目标分辨能力,在快拍数与阵元数相当时改进算法比协方差方法有更小的计算复杂度.仿真分析结果证明了算法的有效性.     

压力负荷所致左室心肌肥大和原癌基因C-myc表达关系的研究

在腹主动脉狭窄所致心肌肥大模型上，分别于２ｈ、８ｈ、１２ｈ、４８ｈ、１ 周、２ 周用形态学计量法（Ｍｏｒｐｈｏｍ ｅｔｒｙ）检测单位体积心肌细胞核数［Ｎ（ｎ）ｖ］和每核平均细胞体积［Ｖ（ｍ ）ｎ］；用核酸原位杂交技术检测原癌基因Ｃ ｍ ｙｃ表达强度。结果显示：手术组与对照组相比，Ｎ（ｎ）ｖ 和Ｖ（ｍ ）ｎ ２ｈ、８ｈ、１２ｈ 时无显著差异（Ｐ＞ ０．０５），４８ｈ、１周、２ 周时，手术组明显大于对照组（Ｐ＜ ０．０５）；原癌基因Ｃ ｍ ｙｃ ２ｈ 开始在手术组左室心肌细胞中表达，８ｈ 达高峰，４８ｈ 消失。提示原癌基因Ｃ ｍ ｙｃ与压力负荷所致左室心肌肥大有密切关系     

连续油管注入头浮动夹头的共振频率测量

国产浮动夹头在注入头高速运行情况下易发生变形.需要检测共振频率,避开易变形区域.采用敲击声频率测量法（以下简称声频法）获取共振频率信号,利用TMS320C6701DSP实现信号的采集并在CCS下编写DSP程序实现HHT分析得到共振频率.试验阶段,通过功率谱分析与HHT对比,得到浮动夹头的各阶固有频率.     

CLUSTER OF WORKSTATIONS BASED ON DYNAMIC LOAD BALANCING FOR PARALLEL TREE COMPUTATION DEPTH-FIRST-SEARCH

IntroductionWith the advantages of the high- speed net-works,cluster of workstations achieve the samescalable parallelism as the current MPPs( Massive-ly Parallel Processors) .But there are many factorssuch as load imbalance,synchronization and com-munication that inhibit good performance in thesesystems.These issues are central to the develop-ment of many parallel applications. Load balanceproblem is a serious impediment to achieving goodperformance in parallel processing the issue of load…     

分界面上基于矢量水听器阵列空间谱DOA估计

矢量水听器通常由三个空间轴向垂直的振速水听器和一个声压水听器构成，可同时测量声信号的振速和声压．为了了解复杂分界面上矢量水听器阵列的定向性能，根据分界面上平面波的反射模型，提出了改进的Bartlett和Capon空间谱，建立了统一框架，并且在绝对硬边界典型界面下，分析了基于矢量水听器阵列的定向性能．给出了具体算例．     

基于调制白噪声与查表法的非平稳路面不平度建模方法

针对实测道路功率谱密度频率结构不同与汽车行驶速度变化引起的非平稳问题, 分析了现有路面不平度模型的准确性, 提出了一种基于调制白噪声与查表法的非平稳路面不平度建模方法; 结合白噪声生成方法和离散功率谱密度的计算公式, 推导出了能够确保路面不平度计算功率谱与设计功率谱完全吻合的Band-Limited White Noise模块参数设定方法; 通过不同设定值组合的白噪声功率谱密度与路面不平度功率谱密度计算结果对比, 验证了所提出的Band-Limited White Noise模块参数设定值的准确性; 为解决频率指数不等于2的空间域内非平稳路面不平度建模问题, 提出了由1个基准项和若干个精度校正项组成, 且调制传递函数模的平方逼近具有任意频率指数的道路功率谱表达式; 为获取考虑车速变化影响的频率时变的时域内非平稳路面不平度输入模型, 基于空间路面不平度固定不变的原理, 提出了利用查表法, 获得变车速工况下时域内非平稳路面不平度; 通过空间域内非平稳路面不平度建模及考虑车速变化的非平稳路面不平度建模应用, 验证了所提建模方法的优越性。研究结果表明: 使用基于调制白噪声的非平稳路面不平度建模方法构建的空间域路面不平度功率谱曲线, 与应用实例中的设计目标曲线吻合良好, 且结合查表法可以有效地解决时变车速带来的时域内非平稳路面不平度建模问题。