交通运输与社会、科学技术的关系研究

交通运输与社会、科学技术之间存在着复杂的关系，它们之间相互影响，相互制约。我国现阶段交通运输与社会、科学技术之间的关系对促进社会发展，改善交通运输供给和提高科学技术水平有着重要作用。     

靖乐渠渠首沉沙池数值模拟及处理效率

甘肃省靖远县靖乐渠由乌兰镇陆家嘴黄河南岸引水,实灌面积33.53 km2,距今已有500余年历史。黄河水挟沙量大,致使下游河道和倒虹吸淤积严重。针对这一问题,拟将原取水口上移3 km,沉沙后引水入城。通过建立沉沙池数学模型,结合引水流量、含沙量、泥沙颗粒级配等多方面因素,对沉沙池沉沙效率、全年淤积厚度及流场分布进行研究,并对两种进口形式的沉沙池方案进行对比分析。结果表明,运用MIKE21泥沙输运模型计算是可行的;沉沙池进口较窄时比方形进口的沉积效果好,流场分布较好,口门处不易堵塞。     

大跨径预应力混凝土连续刚构桥加固施工监控

结合广州市某连续刚构桥加固施工监控实践,介绍了大跨径连续刚构桥加固施工监控的主要内容和重点.该桥加固实测效应和理论计算值较为吻合,说明所介绍的监控方法和措施是有效的,可供同类桥型加固及监测工作借鉴.     

基于组合方法计算权重的绿色铁路客站综合评估

随着我国铁路事业的快速发展,大批的铁路项目在不断建设、改造以及投入运营。在建设绿色铁路客站,推广绿色铁路建筑,避免城市建设造成更多的能源和环境问题,是当前我国城镇化建设必须解决的重大课题。因此,绿色铁路客站的综合评价是非常有必要的。首先建立绿色铁路客站综合评估指标体系;然后采用组合方法计算各评价指标体系的权重;再依据《绿色铁路客站评价标准》的评分准则进行指标打分;最后结合具体实例,计算得出某H铁路客站的综合评价等级。该组合方法的创新之处在于融合专家权重和指标权重为一体,既体现专家自身偏好,同时又克服过于主观的缺点,得出科学、合理的权重,使绿色铁路客站综合评价结果更准确,更具有参考性。     

东京轨道交通直通运营特征分析及启示

通过对东京都市圈轨道交通直通运营情况的系统梳理,整理出不同地铁线路的直通运营互通性。从城市功能分区与城市空间结构两个方面,讨论直通运营线路与城市发展协调性之间的关系。研究早高峰时段轨道交通网络直通运营区段的运力特征,并以京王线-新宿线为例,分析直通运营、快慢车等列车运行组织模式和基本规律,提出对我国轨道交通发展的建议。     

基于MFCM的改进聚类算法及其在交通中的应用

在交通流状态模糊化的过程中,对已有的交通模糊控制研究引入了太多的主观因素.为了解决这个问题,提出了一种基于MFCM算法的分级递减聚类算法,利用MFCM算法寻找类中心,再自适应确定该类中心的隶属度阈值,将聚类进行分级处理,实现未知类别数数据集的聚类.将改进算法应用到交通流状态聚类中,可以更科学地确定交通流状态的聚类数和各类模糊隶属度函数的结构等,最后,通过算例,说明了该算法对于未知聚类数及服从高斯分布的数据集具有聚类效果好、收敛速度快的特点.     

THE STUDY OF MICROSATELLITES ALTERATION IN DIAGNOSES OF BLADDER CANCER

Objective To investigate the possibility of microsatellite alteration （MA） in diagnosis of bladder cancer of Chinese people, and find the better panel which will be used in clinic. Methods A total of 6 and 10 microsatellite markers were chosen, PCR-SSLP silver staining assay was performed in 31 and 32 bladder cancers tissue, exfoliate cells in urine and 10, 15 non-bladder cancers exfoliate cells in urine, respectively. Results MA （＋） was found in 28 out of 31, 30 out of 32 bladder cancers, and the sensitivity was 90. 3%, 93.7% respectively. The MA of urine sediment of 25 non-bladder cancers was negative, and the specificity was 100%. The cytology was carried out among 19 out of 31, 20 out of 32 bladder cancers at the same time, 2 cases （10.3%） and 3 cases （15%） were found cancer positive, and the sensitivity is significantly lower than that by the analysis of MA in exfoliated cells. Conclusion MA was not associated with grade and stage of the bladder cancer. MA assay is a sensitive and effective method for the early detection of bladder cancer and post-operation surveillance.     

一种改良式质粒DNA提取方法的建立与应用

目的建立一种稳定、可靠的质粒DNA提取改良方法,探讨其在分子生物学实验研究中的应用价值。方法将含有目的质粒的菌株扩增后,运用改良后的碱裂解法进行质粒DNA小量提取,微量核酸仪测定提取质粒DNA的产量及纯度;采用琼脂糖凝胶电泳、内切酶酶切及质粒转染真核细胞鉴定提取质粒DNA的质量及转染效率。结果改良式质粒提取方法获得质粒DNA产量为3.2 mg/L菌液,A260/280=1.91;内切酶酶切完全;质粒DNA以超螺旋结构为主;真核细胞转染效率为20%左右。结论改良式质粒DNA抽提方法操作简单、实用,获得质粒DNA产量多、质量高,能达到分子生物学常规实验的要求。     

REAL-TIME DETECTION OF SURVIVIN mRNA EXPRESSION IN CERVICAL CANCER CELL LINES USING MOLECULAR BEACON IMAGING

Objective To detect the expression of survivin mRNA in cervical cancer cell lines using molecular beacon imaging technology. Methods Human cervical cancer cells （HeLa and SiHa） and human fetal lung fibroblast HFL-I were cultured in vitro. After adding 100 nmol/L survivin mRNA molecular beacon, the fluorescent signals were observed under fluorescent microscope. The expressions of survivin in cervical cancer cells and HFL-I cell were examined by immunocytochemical streptravidin-biothin peroxidase （SP） assay at the same time. Results Two kinds of survivin mRNA molecular beacon, with different color fluorescence, had strong fluorescent signal in cervical cancer cell lines, and the signal in SiHa cell line was stronger, but these signals were not found in HFL-I ; Immunocytochemical staining of positive survivin was located in the cytoplasm of cervical cancer cell lines HeLa and SiHa, whereas, no expression of survivin was detected in HFL-I cell line. Conclusion The technology of molecular beacon imaging can be used to detect the expression of survivin mRNA in viable cells successfully, and may provide a new approach to the diagnosis of early stage cervical cancer and the following-up in the clinic.