A NEW METHOD TO CONSTRUCT A FULL-LENGTH cDNA LIBRARY OF HUMAN NORMAL BLADDER TISSUE

ThepurposeoftheHumanGenomeProjectistounravelthe geneticinstructionsencodedbygenes.Identificationof protein codingandfunc tionalsequenceswithin genomeDNAcomplementthephysicalandgeneticmappingwork ,whichlaysfoundationforfurtherresearchintocomplexphe nomenaofgrowth ,development,differentiation ,carcinogenesis,etc.ThesearchforgenesresponsibleforhumandiseasesandbiologicalfeaturesandthestudiesoftheirstructuresandfunctionsrelyontheavailabilityofhighfidelitycDNAlibrariesfromvariouscelltypesandtissu…     

浅谈全球定位系统(GPS)在高速公路测量中的应用

1 GPS测量特点 (1)测站之间无需通视. (2)定位精度高. (3)观测时间短. (4)提供三维坐标. (5)操作简便. (6)全天候作业.     

MICROARRAY ANALYSIS OF DIFFERENT GENE EXPRESSION OF HUMAN CERVICAL CANCER SUBCLONE CELL LINES

Objective To examine the differentially expressed invasion-related genes in two anchorage-independent uterine cervical carcinoma cell lines derived from the same patient using a cDNA array. Methods Two human uterine cervical carcinoma subclonal cell lines CS03 and CS07 derived from a single donor line CS1213 were established by limited dilution procedure. The two cDNA samples retro-transcribed from total RNA derived from CS03 and CS07 cells were screened by a cDNA microarray carrying 234 human cell-cycle related genes and 1011 human signal transduction and membrane receptor -associated genes, scanned with a ScanArray 3000 laser scanner. Results The cDNA microarray analysis showed that ]2 genes in CS03 were up-regulated compared to CS07, and 24 genes in CS07 were upregulated. The function of a number of differentially expressed genes was consistently associated with cell-cycle, cell proliferation, migration, apoptusis, signal transduction and tumor metastasis, including p34^cdc2, TSC22, plasminogen activator inhibitor Ⅰ （PAI-1）and desmusome associated protein（Pinin）. Conclusion Multiple genes are differentially expressed in uterine cervical carcinoma cell lines even came from the same patient. It is suggested that these genes are involved in the different phenotypic characteristics and development of cervical carcinoma.     

RLC－310人体肾透明细胞癌细胞株的生物学和形态学特点

对新建的克隆性人肾透明细胞癌细胞株ＲＬＣ－３１０进行了生物学鉴定和形态学观察。发现细胞可重叠生长，群体倍增时间３２ｈ，集落形成率４４％，具有超二倍体核型，染色体众数６４。细胞没有支原体污染，有特异性的ＬＤＨ同功酶谱，细胞接种Ｂａｌｂ／ｃ裸鼠可形成移植瘤。细胞大小均一，核大，细胞表面有丰富的微绒毛，未见到细胞间的桥粒结构。胞质内有变形的粗面内质网、线粒体和较多糖原颗粒，张力原纤维可见。细胞核异形性明显，核仁数目增多，有边集。表明这株细胞保持了肾透明细胞癌的基本特点。     

柬埔寨金边水净华新桥设计方案介绍

水净华新桥是影响柬埔寨金边市城市出入口通行能力的重要工程,也是中方与柬埔寨政府间的优买合作项目之一.该工程项目由中国路桥工程有限责任公司以设计施工总承包方式组织实施.通过对柬埔寨国情及桥位处地貌、地质、水文及现有桥梁情况的叙述,对新桥桥位和桥型比远方案的论述,介绍了我国设计规范在境外工程的运用情况,展示了一个“走出去”的成功实例.     

转向连接销热处理工艺研究

为避免某重型载货汽车前桥上的转向连接销(40CrH钢)产生淬火裂纹,同时满足金相组织要求,采用水溶性淬火介质(PAG类聚醚)进行了淬火工艺试验.结果表明,采用淬火温度为840℃、水溶性淬火溶液浓度为8％～10％及温度为20～50℃的新工艺进行淬火效果最佳,无淬火裂纹产生,累计生产的50万件转向连接销均未出现质量问题.     

优化的多变量变步长灰色模型及其在路基沉降预测中的应用

传统的单变量或等步长灰色预测模型,未考虑监测点间的系统关联性和实际沉降观测周期,往往具有非等间距的特点,不足以反映路基沉降变形的实际规律。在综合考虑实际工况中断面沉降点间相关性及不等时距观测的基础上,建立路基沉降预测的优化NMGM（1,n）模型。工程实例分析表明,优化的模型与建模工况相适应,具有良好的拟合及预测精度,能够满足工程实际应用需要。     

汽车静电喷涂工艺的改造

上海通用汽车(SGM)金桥南厂油漆车间水性色漆线采用传统的"静电旋杯+空气喷枪(bell+gun)"两道喷涂工艺。第2道空气喷枪喷涂存在喷枪空气帽易堵塞、油漆雾化不均和漆雾飞扬等弊端,导致喷涂效率较低,影响了油漆车间的产能。为此,通过改变喷涂工艺,将"静电旋杯+空气喷枪"转化为"静电旋杯+静电旋杯(bell+bell)",解决了上述问题,满足了油漆车间提高产能的要求。     

带外伸横梁的钢箱梁桥设计实践

当高架桥受桥下河道、道路、地铁、地下管线等限制时,采用外伸横梁是一种有效的解决办法.结合凤凰山高架桥左主线第6联4×28.9 m带外伸横梁的钢箱梁桥,介绍了凤凰山高架桥的设计、计算及施工,并探讨了横梁高度的取值、施工过程中横梁的稳定措施,可为国内外类似桥梁的设计提供参考.     

沥青拌和设备烘干筒内物料烘干加热时间研究

通过综合考虑物料运动过程及高温烟气与物料之间的传热过程,采用简化模型并根据能量守恒原理建立以物料为对象的能量守恒方程,推导出理想条件下的烘干时间计算公式；通过对烘干筒内颗粒状物料运动过程及传热过程的分析,建立了烘干筒内物料烘干加热时间计算公式,计算结果与实际结论吻合良好.