The role of interleukin-8 produced by tumor induced fibroblasts in the development of cutaneous melanoma

Objective To determine the role of interleukin-8 (IL-8) produced by tumor induced fibroblasts in the development of cutaneous melanoma. Methods B16 melanoma cells induced L929 fibroblasts phenotype was transdifferentiated to myofibroblasts (MF) by co-culture in vitro. MF was monitored by morphology and immunophenotype for a-SMA. The level of IL-8 was detected by ELISA. The effect on B16 cell proliferation rate was estimated using MIT method in vitro. Melanoma implanting model was constructed in C57 mice. Results L929 MF phenotype could be modulated by B16 melanoma cells-derived transforming growth factor-β1 (TGF-β1) and elevated the levels of IL-8. L929 MF did not influence the B16 melanoma cells viability in vitro, but shortened the time of tumor formation and increased the incidence rates of tumors in C57 implanting model mice. Conclusion Fibroblasts can be activated by tumor cells and produce IL-8, which acts as an inflammatory cytokine promoting the development of cutaneous melanoma.     

改进不等时距GM模型在施工控制中的应用

刚构桥施工过程中应力变化复杂,影响因素多,较难预测把握应力发展趋势,实测应力缺失时,单一使用GM(1,1)模型预测精度低,对原始应力数据要求苛刻.文章改进了不等时距GM(1,1)模型,结合罗天乐特大桥工程进行验证,结果表明,改进不等时距GM(1,1)模型在刚构桥施工应力预测中具有较好的适用性和较高的预测精度.     

转化生长因子-β_1抗体对人翼状胬肉成纤维细胞增殖及TGF-β_1-Smad4信号转导通路的影响

目的探讨转化生长因子-β1(TGF-β1)抗体对体外培养的翼状胬肉成纤维细胞增殖的抑制作用及对TGF-β1-Smad4信号转导通路的影响,评估其在翼状胬肉发病机制中的作用。方法人翼状胬肉切除后,体外培养其成纤维细胞并传至4~6代时,分别用6.25、12.5、25、50mg/mL TGF-β1抗体处理24、48、72h,采用MTT法检测成纤维细胞的生长抑制率,RT-PCR检测TGF-β1mRNA表达,Western blot检测Smad4蛋白的表达。所得数据采用SPSS19.0统计软件处理。结果 TGF-β1抗体可不同程度抑制成纤维细胞的增殖;TGF-β1抗体处理组TGF-β1mRNA的表达明显低于对照组,且不同浓度组间差异有统计学意义(P<0.05),随着浓度增大和作用时间延长,TGF-β1抗体处理组TGF-β1 mRNA的表达量呈逐渐降低趋势。不同浓度的TGF-β1抗体对Smad4的蛋白表达具有明显增强作用,且各组间差异有统计学意义(P<0.05)。结论 TGF-β1抗体对人翼状胬肉体外培养的成纤维细胞生长具有明显抑制作用,其可能通过调节TGF-β1和Smad4的表达而实现。     

吸烟和β3-肾上腺素能受体基因Trp64Arg、锰超氧化物歧化酶9Ala/Val基因多态性与非酒精性脂肪性肝病的相关性

目的探讨吸烟和β3-肾上腺素能受体(β3-AR))基因Trp64Arg、锰超氧化物歧化酶9Ala/Val(MnSOD9Ala/Val)基因多态性与非酒精性脂肪性肝病(NAFLD)发病之间的关系。方法采用病例-对照研究的方法,以720例NAFLD患者及720例健康对照者的外周血白细胞为样本,采用聚合酶链反应(PCR)技术分析β3-AR基因Trp64Arg和MnSOD9Ala/Val基因多态性。结果β3-AR基因Trp64Arg(A/A)基因型和MnSOD9Ala/Val(V/V)基因型频率分布分别为39.4%、71.7%(病例组)和21.1%、43.3%(对照组),差异有统计学意义(P<0.01;P<0.01)。Trp64Arg(A/A)基因型者患NAFLD的风险显著增加(OR=2.434,95%CI=1.816~4.075)。MnSOD9Ala/Val(V/V)基因型者患NAFLD的风险也显著增加(OR=3.308,95%CI=1.913~4.509)。基因突变的协同分析发现,Trp64Arg(A/A)/MnSOD9Ala/Val(V/V)基因型者在NAFLD组和对照组中的分布频率分别为32.8%和6.5%,差异有统计学意义(P<0.01)。Trp64Arg(A/A)/MnSOD9Ala/Val(V/V)基因型者患NAFLD的风险显著增加(OR=9.753,95%CI=4.292~12.426)。病例组的吸烟率显著高于对照组(OR=2.623,95%CI=1.425~4.957),Trp64Arg(A/A)/MnSOD9Ala/Val(V/V)基因型与吸烟有协同作用(OR=33.764,95%CI=18.907~61.582)。结论 Trp64Arg(A/A)/MnSOD9Ala/Val(V/V)基因型和吸烟是NAFLD的易患因素,三者的联合在NAFLD的发生中起着协同的作用。     

船舶非线性横摇运动数值模拟研究

非线性大角横摇运动是危害船舶安全的重要因素,也是船舶水动力学关注的热点及难点问题。文章采用CFD方法,对某型驱逐舰船模在不同波长规则波中横浪航行时的横摇运动进行了数值模拟。通过与水池实验比对,数值仿真得到的稳定横摇幅值与实验值的误差在10%左右。同时,数值模拟证实了非线性横摇的两种典型特征—“多值”及“跳跃”现象。数值模拟结果表明,CFD方法可作为研究船舶非线性横摇运动的有效手段。     

Efficient missing data imputing for traffic flow by considering temporal and spatial dependence

The missing data problem remains as a difficulty in a diverse variety of transportation applications, e.g. traffic flow prediction and traffic pattern recognition. To solve this problem, numerous algorithms had been proposed in the last decade to impute the missed data. However, few existing studies had fully used the traffic flow information of neighboring detecting points to improve imputing performance. In this paper, probabilistic principle component analysis (PPCA) based imputing method, which had been proven to be one of the most effective imputing methods without using temporal or spatial dependence, is extended to utilize the information of multiple points. We systematically examine the potential benefits of multi-point data fusion and study the possible influence of measurement time lags. Tests indicate that the hidden temporal–spatial dependence is nonlinear and could be better retrieved by kernel probabilistic principle component analysis (KPPCA) based method rather than PPCA method. Comparison proves that imputing errors can be notably reduced, if temporal–spatial dependence has been appropriately considered.     

The visual impact of development in coastal zone areas

Abstract

The focus of this paper is theoretical, as well as methodological. It reviews previous studies of visual impact and reports research by the author on people's evaluation of the appropriateness of different man‐made facilities in different coastal‐area contexts and other environments. In this study, subjects were asked to react to slides developed through the use of landscape models and experimental facilities to simulate the appearance of a building in the landscape, while systematically varying the visual relatedness between the two. Specific interest centered on the variable of congruity vs. contrast between the appearance of the building and its landscape context, manipulated by co‐varying the attributes of color and size to create a five‐point scale of contrast/obtrusiveness. The hypothesis was that judgments of appropriateness, and to a lesser extent judgments of liking, would vary inversely with level of contrast. The role of two further variables, the functional significance of the building in its setting, and the character of the setting itself, was also investigated. The author reviews methodological issues involved where subjects respond to visual stimuli for purposes of judging change in visual quality. These methodological issues include: stimulus‐selection and problems of simulation, choice of response measures, and choice of respondents, and the treatment of individual differences. The author calls for development of standardized scales and indices such as “perceptually‐relevant environmental quality indicators.”     

Short-term forecasting of high-speed rail demand: A hybrid approach combining ensemble empirical mode decomposition and gray support vector machine with real-world applications in China

Short-term forecasting of high-speed rail (HSR) passenger flow provides daily ridership estimates that account for day-to-day demand variations in the near future (e.g., next week, next month). It is one of the most critical tasks in high-speed passenger rail planning, operational decision-making and dynamic operation adjustment. An accurate short-term HSR demand prediction provides a basis for effective rail revenue management. In this paper, a hybrid short-term demand forecasting approach is developed by combining the ensemble empirical mode decomposition (EEMD) and grey support vector machine (GSVM) models. There are three steps in this hybrid forecasting approach: (i) decompose short-term passenger flow data with noises into a number of intrinsic mode functions (IMFs) and a trend term; (ii) predict each IMF using GSVM calibrated by the particle swarm optimization (PSO); (iii) reconstruct the refined IMF components to produce the final predicted daily HSR passenger flow, where the PSO is also applied to achieve the optimal refactoring combination. This innovative hybrid approach is demonstrated with three typical origin–destination pairs along the Wuhan-Guangzhou HSR in China. Mean absolute percentage errors of the EEMD-GSVM predictions using testing sets are 6.7%, 5.1% and 6.5%, respectively, which are much lower than those of two existing forecasting approaches (support vector machine and autoregressive integrated moving average). Application results indicate that the proposed hybrid forecasting approach performs well in terms of prediction accuracy and is especially suitable for short-term HSR passenger flow forecasting.     

二赛线GTM沥青混合料设计

结合内蒙古自治区二连浩特-赛汉塔拉的工程实例,介绍了GTM混合料设计方法的特点,以及根据当地的气候、交通状况确定工程级配范围和室内采用GTM完成混合料的设计及技术性能的检验。     

GTM法在公路沥青路面施工中的应用研究

结合GTM法技术在河北省张石高速公路中的实践应用,介绍GTM法的设计原理、施工工艺和质量控制体系,尤其是对施工过程质量控制点和沥青路面最大密实度标准的研究分析,有效地降低了工程施工费用,提高了路面的服务寿命。