Relationship between the expression of matrix metalloproteinase-9 and angiogenesis in glioma and its clinical significance

Objective To explore the role and significance of matrix metalloproteinase-9 (MMP-9) in angiogenesis through observing the relationship between the expression of MMP-9 and microvessel density (MVD) in glioma. Methods The expressions of MMP-9 and CD34 in 10 cases of normal brain tissues and 58 cases of glioma (14 cases of grade Ⅰ , 20 cases of grade Ⅱ , 15 cases of grade Ⅲ, and 9 cases of grade Ⅳ ) were detected by immunohistochemical streptavidin-peroxidase technique. The positive cells of MMP-9 and the positive microvessels were examined under binocular light microscope. Results The positive expression of MMP-9 in glioma was located in the tumor-cell cytoplast and endothelial cells. The positive rate of MMP-9 in glioma of grade Ⅰ, Ⅱ, Ⅲ and Ⅳ was 42.9%, 65.0%, 86.7% and 88. 9%, respectively. The expression of MMP-9 was obviously higher than that of normal brain tissues (P<0.01) and positively correlated with glioma malignancy (rz =0. 597, P<0.05). MVD was correlated with glioma malignancy (H=47. 865, P<0. 05). The expression of MMP-9 was significantly correlated with MVD (rz =0.897, P<0.01). Conclusion The expressions of MMP-9 and MVD are correlated with glioma malignancy, which may be helpful in judging the malignancy, invasion and prognosis. MMP-9 plays an important role in angiogenesis of glioma and accelerates glioma malignancy development by promoting angiogenesis.     

新型相离型曲线尖轨9号道岔的扳动力分析

采用有限元方法,建立新型相离型曲线尖轨9号道岔的扳动力计算模型,分析摩擦系数、尖轨动程、尖轨与滑床板密贴、道岔轨底坡等取决于安装及维修状态的因素,以及对尖轨扳动力和不足位移的影响.提出在铺设及养护维修过程中应注意的事项.探讨9号道岔取消第2牵引点的可行性,认为在目前的尖轨结构下保持2点牵引较为可靠.     

管片预埋滑槽在深圳地铁9号线的应用

管片预埋滑槽作为一项新型技术,在深圳市地铁9号线全线引入和应用,替代传统预埋工艺,为盾构区间隧道内设备及管线提供安装基础。介绍管片预埋滑槽技术的优点和特性,利用Abaqus 6.10程序仿真验证盾构管片满足载荷要求。在动态与静态两种工况下,对刚性悬挂接触网、疏散平台及管道等载荷进行受力分析,得出预埋滑槽满足其受力要求;计算预埋滑槽和T型螺栓的承载力,证实预埋滑槽满足安装受力要求。     

地铁9号曲尖轨道岔区建筑限界加宽量计算

限界计算是地铁工程设计过程中较为繁琐的一项工作,直接关系到车辆运行安全。针对天津地铁6号线工程中应用9号曲尖轨道岔的情况,综合考虑车辆在道岔侧股运行时的几何偏移量、欠超高引起的动态偏移量、曲线轨道参数及车辆参数变化引起的车体横向位移量,计算并拟合B型车道岔区建筑限界加宽量图,为道岔区土建、结构设计提供理论依据。计算结果表明:B型车道岔外侧建筑限界加宽始于岔心前端25.75m处,最大加宽量为166 mm;道岔内侧加宽始于岔心前端22.55 m处,最大加宽量为429 mm。     

9机牵引道岔控制电路分析

分析了9机牵引道岔控制电路,阐述了9机牵引单动、双动道岔的启动过程及故障情况下的安全防护措施,并结合实际使用过程中出现的问题与现象提出可行的解决方案。     

微血管密度和基质金属蛋白酶-9的表达与胶质瘤病理分级的关系

目的　探讨微血管密度 (MVD)和基质金属蛋白酶 9(MMP 9)的表达与人脑胶质瘤恶性程度及预后的关系。方法　采用免疫组织化学SP法检测 5 8例不同病理分级的人脑胶质瘤及 10例正常脑组织标本中MMP 9和CD3 4 的表达 ,测定其阳性细胞数和阳性血管数。结果　胶质瘤中MMP 9表达于肿瘤细胞胞浆和血管内皮细胞 ;不同病理分级胶质瘤中MMP 9阳性表达率分别为Ⅰ级 4 2 .9% ,Ⅱ级 6 5 .0 % ,Ⅲ级 86 .7% ,Ⅳ级 88.9% ,显著高于正常脑组织 (P<0 .0 1) ,且与胶质瘤恶性程度呈正相关 (rs=0 .5 97,P <0 .0 5 ) ;不同病理分级胶质瘤中MVD存在显著性差异 (H =4 7.86 5 ,P <0 .0 5 )。结论　MVD和MMP 9的表达与胶质瘤恶性程度密切相关 ,可作为临床判断胶质瘤恶性程度、侵袭性及预后的重要指标。     

中药治疗后MMP-9、TIMP-1及CA125在异位和在位子宫内膜中的表达

目的 探讨中医药治疗子宫腺肌症的作用机制.方法 37例行全子宫切除术的子宫腺肌症患者术前分为中药治疗组(n=17)和对照组(n=20).子宫内膜作为在位内膜标本,腺肌瘤作为异位内膜标本.采用免疫组化法测定并比较在位和异位内膜中基质金属蛋白酶-9(MMP-9)和组织金属蛋白酶抑制剂-1(TIMP-1)的表达水平,并观察两组患者治疗前、后血清CA125的变化.结果 中药治疗组异位内膜组织中,MMP-9在腺上皮细胞内的表达显著低于对照组(P<0.05),TIMP-1的表达显著高于对照组(P<0.05),MMP-9/TIMP-1比值显著低于对照组(P<0.05).中药组治疗后血清CA125 明显降低,与治疗前及对照组比较差异均有统计学意义(P<0.05).结论 中药可能通过下调MMP-9/TIMP-1的比值及降低血清CA125水平来控制异位内膜组织的种植侵袭.     

Construction of expression vector for NT4-ADNF-9 fusion gene

Objective To construct the prokaryotic expression vector bearing fusion gene NT4-ADNF-9 and lay foundation for further study on genetic therapy of neuraseusory deafness. Methods By means of asymmetrical prince/ template, double stranded eDNA of activity dependent neurotrophic factor-9 (ADNF-9) was obtained, which included restriction enzymes sites on the two extremities. ADNF-9 eDNA was ligated to the signal and leader peptides of nenrotrophin 4 (NT4), and the fusion gene was named NT4-ADNF-9. Then it was suheluned into prokaryotic expression vector pBV220, and called pBV220/ NT4-ADNF-9. Results Evidences of DNA sequence analysis and restrtction enzymes digestion showed that we recombined ADNF-9 eDNA to the 3'terminal of the signal and leader peptides of NT4, and the fusion gene was subcluned into pBV220 successfully. Bioactivity of the products was proved that it could support the cell survival and neurite growth in the primary cultures of dorsal root ganglia (DRG) of embryonic day-8 cbicken neurons as compared to the control. Conclusion Prokaryotic expression vector pBV220/NT4-ADNF-9 can be constructed successfully and the bioactivtty is satisfactory.     

基于汽车ECU串口的代码下载与调试方法的实现

提出一种基于串口通信来实现对汽车电控单元ECU代码的下载和调试方法,以解决长期以来依赖BDM对ECU进行代码下载和调试过程中出现的诸多不便.以美国飞思卡尔汽车级微控制芯片HCS 9S12为研究对象,通过自主开发的调试软件实现了无BDM对ECU的代码下载与调试,实践证明该方法简单、可靠、成本低,有一定的应用推广价值.     

DF9型客运内燃机车电传动系统改造设计

简要介绍了DF9型客运内燃机车运用情况,详细介绍了机车改造以后电传动系统的主要技术参数及特点。改造后的机车经过了负载试验及正线试运的考核,目前运用情况良好。