Insertion Mutagenesis of Trichoderma atroviride by Restriction Enzyme-mediated DNA integration

The integration of transforming DNA mediat-ed by the in vivo action of restriction enzymes(RE-MI) was first described in yeast. Insertion mutage-nesis by REMI is a method that is being used togenerate mutations whose molecular basis can beeasily identified. REMI mutagenesis has a majoradvantage over classical mutagenesis in that themutants are marked with a molecular tag by themutagenic event. REMI has been described inmany organisms including the filamentous fungi,and its technology ha…