边界条件对吊索索力估算的影响

从Bernoulli—Euler理论出发，推导了吊索在一般边界条件下的自由振动频率方程和索力的解析表达式。分析了边界条件对吊索自振频率的影响，并与有限元结果进行了比较。结果表明，边界条件对吊索索力估算的影响不可忽略。     

小流域水文计算的分析与应用

本文结合工程实例,介绍了小流域的设计流量计算方法—径流形成法。这种方法对相关的勘测设计中的流量计算具有参考意义。     

Toxicity to the normal hemocytes by ALA-PDT for the ex vivo purging of hematopoietic stem cell grafts

Objective To study the toxic effects of 5-amionlevulinic acid-based photodynamic therapy (ALA-PDT) on human peripheral blood mononuclear cells (PBMCs), cord blood mononuclear cells (CBMCs) and peripheral blood stem cells (PBSCs), and furthermore, to understand the possible causes of this response. Methods We used MTT assay to detect the survival rate of PBMCs, CBMCs and PBSCs after treated by ALA-PDT under the optimum experiment conditions with U937 as control;Annexin V-FITC/PI was used to detect the pattern of cell death induced by ALA-PDT. By using flow cytometry, we detected intracellular PpIX fluorescence intensity. Results After ALA-PDT treatment the survival rate of PBMCs had no significant change;however in PBSCs and CBMCs, the survival rate reduced to 70%, and the survival rate of leukemia cell U937 was the lowest, about 30%. After incubation with ALA,except for PBMCs, intraceUuiar PplX fluorescence intensity of the other two kinds of normal haemocytes and U937 increased obviously. These results combined with the flow cytometry suggested that the main pattern of cell death here was apoptosts. Conclusion Under the optimum experiment conditions, ALA-PDT has a slight effect on normal haemocytes but excellent depletions of leukemia cells. Therefore, it can effectively purify autologons bone marrow or stem cell grafts.     

基于非线性最小二乘的短线法节段预制线形控制研究

对于采用短线法节段预制拼装施工的预应力钢筋混凝土桥,节段预制过程中较小的误差都可能导致成桥线形较大的偏差。为控制短线法节段预制过程中产生的误差,以嘉绍大桥北岸引桥（为13联70 m跨预应力混凝土连续刚构桥,主梁为单箱双室斜腹板箱梁,采用短线法节段预制拼装施工）为背景,在对预制线形控制原理研究的基础上,提出基于非线性最小二乘的综合误差处理方法,并编制线形控制系统BSRI＿SLCS。该方法首先计算理论预制线形,其次建立预制线形整体坐标系与节段局部坐标系,实现节段控制点坐标在不同坐标系中的变换,然后根据实测数据进行误差分析,调整匹配节段的位置。该方法在嘉绍大桥北岸引桥短线法施工中应用结果显示,成桥线形与理论线形较为接近,证明该方法对预制线形控制是可行的。     

大跨度隧道下穿敏感高压电塔施工结构稳定控制研究与应用

在地表上有类似高压输电铁塔的区域开挖大跨度隧道,如何最大限度的减少对地表结构物的扰动,保证此类结构物的安全是隧道施工中必须解决的问题。依据3个支撑点就能保证结构稳定的原理,提出了李家冲隧道穿越高压电塔的施工方案,且该方案在实际施工过程中效果良好。