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The integration of transforming DNA mediat-ed by the in vivo action of restriction enzymes(RE-MI) was first described in yeast. Insertion mutage-nesis by REMI is a method that is being used togenerate mutations whose molecular basis can beeasily identified. REMI mutagenesis has a majoradvantage over classical mutagenesis in that themutants are marked with a molecular tag by themutagenic event. REMI has been described inmany organisms including the filamentous fungi,and its technology ha… 相似文献
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Cucumber powdery mildew disease caused bySphaerotheca fuliginea is a serious disease of culti-vated cucumber in most cucumber-growing regionsof the world. Understanding resistant mechanismof cucumber against powdery mildew disease is in-creasingly crucial for breeding resistant cultivars toeffectively overcome the disease. In the case, agreat deal of researches on single proteins or en-zymes encoded by the resistant genes have beenperformed to uncover defense mechanism. Howev-er, so far there … 相似文献
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