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Globally significant quantities of organic carbon are stored in northern permafrost soils, but little is known about how this carbon is processed by microbial communities once it enters rivers and is transported to the coastal Arctic Ocean. As part of the Arctic River-Delta Experiment (ARDEX), we measured environmental and microbiological variables along a 300 km transect in the Mackenzie River and coastal Beaufort Sea, in July–August 2004. Surface bacterial concentrations averaged 6.7 × 105 cells mL− 1 with no significant differences between sampling zones. Picocyanobacteria were abundant in the river, and mostly observed as cell colonies. Their concentrations in the surface waters decreased across the salinity gradient, dropping from 51,000 (river) to 30 (sea) cells mL− 1. There were accompanying shifts in protist community structure, from diatoms, cryptophytes, heterotrophic protists and chrysophytes in the river, to dinoflagellates, prymnesiophytes, chrysophytes, prasinophytes, diatoms and heterotrophic protists in the Beaufort Sea.Size-fractionated bacterial production, as measured by 3H–leucine uptake, varied from 76 to 416 ng C L− 1 h− 1. The contribution of particle-attached bacteria (> 3 µm fraction) to total bacterial production decreased from > 90% at the Mackenzie River stations to < 20% at an offshore marine site, and the relative importance of this particle-based fraction was inversely correlated with salinity and positively correlated with particulate organic carbon concentrations. Glucose enrichment experiments indicated that bacterial metabolism was carbon limited in the Mackenzie River but not in the coastal ocean. Prior exposure of water samples to full sunlight increased the biolability of dissolved organic carbon (DOC) in the Mackenzie River but decreased it in the Beaufort Sea.Estimated depth-integrated bacterial respiration rates in the Mackenzie River were higher than depth-integrated primary production rates, while at the marine stations bacterial respiration rates were near or below the integrated primary production rates. Consistent with these results, PCO2 measurements showed surface water supersaturation in the river (mean of 146% of air equilibrium values) and subsaturation or near-saturation in the coastal sea. These results show a well-developed microbial food web in the Mackenzie River system that will likely convert tundra carbon to atmospheric CO2 at increasing rates as the arctic climate continues to warm.  相似文献   
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冰脊和冰山与极地船体发生碰撞将影响船体结构安全,并决定船体结构设计载荷。船体结构设计应遵循基于风险的设计原则,通常涉及以下极限状态设计准则:1)服务极限状态(SLS);2)最终极限状态(ULS);3)疲劳极限状态(FLS);4)事故极限状态(ALS)。最终极限状态和事故极限状态对应于发生概率极低的船与冰脊作用以及冰山撞击事件,旨在确保船体结构不会完全损毁。然而,对于较低概率水平的冰载荷分析,需进行较大数据量的工况分析,计算耗时无法承受。为此,引入环境等值轮廓方法,来大幅减少冰载荷分析所需工况数量,并给出设计工况最有可能的冰体参数组合方式。结合最终极限状态和事故极限状态,举例说明这一方法的运用方式。环境等值轮廓方法可为现有极地船舶船体结构设计方法提供有效补充。  相似文献   
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The paper presents a simple procedure to estimate the damage to a ship bottom and the associated seabed topology that results from a dynamic grounding event. The seabed is modeled as a rigid body and parameterized by a quadratic surface, i.e., a paraboloid, which can in principle model a wide range of seabed topologies. A nonlinear finite element program (LS-DYNA) is used to simulate the contact force versus the lateral penetration, from which the horizontal force component of powered grounding is estimated. The simplified procedure for analyzing dynamic and static grounding events is outlined. Simulations are performed for different ship speeds and for different initial levels of obstruction over the keel. It is shown that a static approach may replace the dynamic grounding simulation, thereby considerably reducing the computational work. The static approach allows for the quick estimation of the energy absorption during powered grounding, which is imperative for decision making during critical situations. The ultimate goal of the study is to provide a near real-time prediction of the risk of rupture of the cargo tanks and hull girder failure. Moreover, the residual strength of damaged ships is an important issue that is related to operations involved in the salvage of wrecked vessels, such as re-floatation and towing.  相似文献   
4.
Results from four different methods for stochastic dynamic response analysis of a proposed jack-up platform are compared. This structure exhibits both significant dynamic amplification and non-linear transfer of sea elevation into load effects. Both estimation of extreme response and fatigue damage are considered. The most complex procedure based on time-domain simulation and step-by-step integration is employed as a benchmark for assessment of three simplified methods. The simplifications consist of various types of linearization in conjunction with transfer function approximations. Applicability of the methods to structures with increasingly non-linear behaviour and dynamic amplification is briefly discussed.  相似文献   
5.
This study proposes a new procedure for the estimation of stranding forces and their contact positions. The method is based on the measurement of a few characteristic on-site parameters, i.e., the draughts and bending moments acting on a stranded ship. A procedure that estimates penetration into the ship bottom based on knowledge of the resistance versus penetration relationship is also presented. The seabed topology is parameterized by a paraboloid. This geometry can, in principle, characterize a wide range of obstructions, from sharp rocks to large shoals. Nonlinear finite element analysis is used to simulate various stranding situations and to generate the corresponding resistance versus penetration curves. The present method provides insight into the identification of real stranding scenarios in terms of the location of obstructions, their possible shapes and the corresponding resistance-penetration curves. The proposed methodology represents a first step towards a tool for quick decision making during salvage operations. The ultimate goal is to allow near real-time prediction of the risk of penetration into cargo tanks and hull girder failure. To demonstrate the effectiveness of the proposed method, it is applied to a simulated trial stranding scenario.  相似文献   
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