Role of candesartan against cerebral ischemia and oxidative damage in normotensive rats

Objective Angiotensin Ⅱ (Ang Ⅱ ) contributes to modulating blood pressure by stimulation of Ang Ⅱ AT1 receptors. We devised a rat transient middle cerebral artery occlusion (MCAO) model to assess whether oxidative damage is decreased after pretreatment with Angiotensin Ⅱ AT1 receptor blocker (ARB). Methods After 2 weeks pretreatment with ARB 0. 5 and 1 mg/kg, the male Wister rats were subjected to 2 h middle cerebral artery occlusion (MCAO). At 24 h, the lumen diameter of middle cerebral artery, the plasma level of 8-hydroxy-2'-deoxyguanosine (8-OHdG), and HIF-1 a levels were recorded and compared. Results After pretrcatment with ARB 0.5 and 1 mg/kg, blood pressure did not significantly change compared with that of controls. In the group of candesartan at 1 mg/(kg· day), the lumen diameter was significantly increased compared to that in control group [(86.0±5.0) μm vs. (69.0± 2.1) μm; P<0. 01, n = 6- 8]. The plasma 8-OHdG levels of ARB pretreatment groups were decreased. In immunohistochemical findings, 8-OHdG- and HIF-1α-containing cells in ARB pretreatment groups were decreased. Conclusion Brain ischemia and oxidative damage can be reversed by AT1 receptor blockade in normotensive rats after transient cerebral artery occlusion.     

Java优化策略在铁路互联网售票系统中的应用研究

为了使铁路互联网售票系统稳定性和高效性能够得到更好的保证，结合分布式内存数据库的系统特性和铁路互联网售票系统的业务特性，对各种Java虚拟机（JVM）内存管理策略和垃圾回收机制进行了分析和研究，以余票查询业务系统模块为例对JVM参数调优过程进行了简要测试和分析，测试结果表明，优化后的系统具有更好的稳定性和高效性。     

汽车技术状况在行车安全中的作用

汽车技术作为行车安全的保障,加大汽车技术的研究可以提高行车的安全性,减少安全事故的发生。本文就汽车技术状况在行车安全中的作用进行了相关的分析。     

慢性硬膜下血肿术后引流量的临床意义探讨

慢性硬脑膜下血肿 (ｃｈｒｏｎｉｃｓｕｂｄｕｒａｌｈｅｍａｔｏｍａ ,ＣＳ ＤＨ)是中老年患者轻微颅脑损伤后最为常见的颅内病变。虽然ＣＳＤＨ的治疗方法多种多样 ,但钻孔闭式引流术已成为神经外科医生普遍采用的方法。我们对ＣＳＤＨ患者术后引流量进行了对比观察 ,现将结果报告如下。1　资料和方法选择 1991年 1月至 2 0 0 0年 12月住本院神经外科资料完整的ＣＳＤＨ患者 15 2例。其中男 138例 ,女 14例 ,年龄 36～ 82岁 ,平均 6 2 .3岁。 5 0～ 6 5岁者 12 6例占 83% ;双侧ＣＳＤＨ者 2 3例。 15 2例ＣＳＤＨ患者术前均行头颅ＣＴ扫描…     

基于大数据技术的铁路互联网售票异常用户行为分析研究与实现

近几年铁路互联网售票系统不断完善，给人民群众的出行带来了很大的便利，售票量不断增加，同时也存在抢票、囤票等异常用户行为，为了保障售票系统的安全稳定运行及维护公平公正的售票环境，提出了基于大数据技术的海量用户行为日志分析系统架构，有效地识别出异常购票行为。     

互联网历史订单集群的设计与实现

针对历史订单的查询流程,将内存数据库技术和大数据Hadoop技术无缝结合,实现旅客快速地查询到更多的历史订单信息。不仅为旅客查询订单提供便利,也为分布式大数据技术在客票系统中的广泛推广打下坚实的基础。     

东营市五六干合排污水处理厂工艺设计

东营市五六干合排污水处理厂是东营市第一座设计出水标准达到类Ⅳ类水体标准的市政污水处理厂。一期工程设计规模2.5×10^4 m^3/d,采用“预处理+巴颠甫生物池+高效沉淀池+反硝化滤池+浸没式超滤”工艺,消毒采用紫外线消毒工艺。详细介绍了该工程处理工艺的设计情况,对关键技术进行了说明。该工程自2016年底建成调试完成以来,运行出水水质稳定达标。     

siRNA抑制hTERT对脑胶质瘤T98G细胞增殖及周期的影响

目的研究人端粒酶逆转录酶被抑制后胶质瘤细胞系体外增殖及周期的变化,以期寻找控制胶质瘤细胞恶性行为的有效途径。方法人工合成小干扰RNA(siRNA)序列,并将其通过lip2000转入体外培养的T98G胶质瘤细胞系中,荧光显微镜观察转染效率,免疫印记检测蛋白表达情况,MTT法检测细胞体外增殖表现,绘制生长曲线图,流式细胞仪检测细胞周期的变化。结果成功转染siRNA,并有效抑制人端粒酶逆转录酶的活性,免疫印记验证干扰效果可靠,体外可抑制胶质瘤细胞的增殖,与对照组比较其抑制效率的差异有统计学意义(P<0.05)。结论体外条件下,可以通过siRNA有效抑制人端粒酶逆转录酶,从而有效抑制胶质瘤细胞的体外增殖,使胶质瘤细胞周期发生变化。这可能为胶质瘤的治疗带来新的启示。     

茚甲新对胶质瘤细胞体外侵袭能力的影响

目的 研究茚甲新对胶质瘤细胞体外侵袭能力的抑制作用.方法 通过MTT实验确定不会引起细胞毒性的茚甲新药物浓度,应用移行实验和浸润实验研究茚甲新对胶质瘤细胞移行及浸润能力的影响.结果 在一定的浓度范围内,随着茚甲新浓度的增加,胶质瘤细胞的浸润能力明显下降,并且与对照组相比有统计学意义(P＜0.05).但是,其浓度并不影响...     

Relationship between the expression of matrix metalloproteinase-9 and angiogenesis in glioma and its clinical significance

Objective To explore the role and significance of matrix metalloproteinase-9 (MMP-9) in angiogenesis through observing the relationship between the expression of MMP-9 and microvessel density (MVD) in glioma. Methods The expressions of MMP-9 and CD34 in 10 cases of normal brain tissues and 58 cases of glioma (14 cases of grade Ⅰ , 20 cases of grade Ⅱ , 15 cases of grade Ⅲ, and 9 cases of grade Ⅳ ) were detected by immunohistochemical streptavidin-peroxidase technique. The positive cells of MMP-9 and the positive microvessels were examined under binocular light microscope. Results The positive expression of MMP-9 in glioma was located in the tumor-cell cytoplast and endothelial cells. The positive rate of MMP-9 in glioma of grade Ⅰ, Ⅱ, Ⅲ and Ⅳ was 42.9%, 65.0%, 86.7% and 88. 9%, respectively. The expression of MMP-9 was obviously higher than that of normal brain tissues (P<0.01) and positively correlated with glioma malignancy (rz =0. 597, P<0.05). MVD was correlated with glioma malignancy (H=47. 865, P<0. 05). The expression of MMP-9 was significantly correlated with MVD (rz =0.897, P<0.01). Conclusion The expressions of MMP-9 and MVD are correlated with glioma malignancy, which may be helpful in judging the malignancy, invasion and prognosis. MMP-9 plays an important role in angiogenesis of glioma and accelerates glioma malignancy development by promoting angiogenesis.