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西安汉族人群HLA-DQA1基因座遗传多态性研究   总被引:2,自引:0,他引:2  
目的 了解西安汉族群体HLA DQA1基因座的基因及基因型分布 ,获得西安汉族群体HLA DQA1群体遗传学数据。方法 采用PERKIN ELMER公司生产的AmpliTypePM试剂盒 ,PCR扩增HLA DQA1基因座 ,与 11个寡核苷酸探针杂交 ,对西安地区汉族 10 2名无关个体HLA DQA1基因座进行基因频率调查。结果 检出 7种等位基因、2 8种基因型 ,经 χ2 检验基因型的观察值与期望值符合Hardy Weinberg平衡定律 (P >0 .5 ) ,其杂合度 (H)、个体识别率 (DP)、非父排除率 (EPP)和多态信息量 (PIC)分别为 0 .8317、0 .9393、0 .786 6和0 .82 5 0。结论 所得到的群体遗传学数据为遗传学、法医学个体识别和亲权鉴定提供依据  相似文献   
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云南白族STR遗传多态性研究   总被引:4,自引:1,他引:3  
目的 研究我国白族STR遗传多态性。方法 通过STR复合扩增、基因扫描、基因分型调查了 98名中国白族无关个体 1 5个STR基因座等位基因分布情况。结果 共检出 1 3 4个STR等位基因 ,其频率分布在 0 .0 0 5 8~ 0 .5 799之间 ,杂合度 (H)为 0 .5 83 4~ 0 .882 8,个体识别力 (DP)为 0 .773 9~ 0 .9666,非父排除率 (EPP)为 0 .5 692~ 0 .8694,多态信息量 (PIC)为 0 .5 3 1 7~ 0 .8694。结论 为进一步研究中华民族STR遗传结构奠定了基础 ,在人类学、法医学等领域也有重要的应用价值  相似文献   
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A NORTHWEST DATABASE MODEL OF SHORT TANDEM REPEAT LOCI IN FORENSIC MEDICINE   总被引:1,自引:0,他引:1  
CriminalratewasrisingyearafteryearinthenorthwestChina .Itisnecessarytoestablishthefo rensicDNAdatabase .Thereweremanyshorttan demrepeats (STRs)thatappearedtobeabundantandoccurred 15 - 2 0kbonaverageinthehumange nome .STRlociconsistedof 2 - 6bpinlengthasitscoresequences[1] .Thecharacteristicsofhighlyeffi cientandmultipleamplificationmadeSTRsuperiortothetraditionalgeneticmarkers .Alloftheselect edSTRlocihadcommonalleleslessthan 35 0bpinsize[2 ] .STRwasthemostidealgeneticmarkerbe causeofi…  相似文献   
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STRisauniversalgeneticmarkerthathaschangeable polymorphismandstableheredityinhuman genome .ItisaspecificDNAsegmentcomposedof 2~ 7basepairsasitscoresequence ,andisformedthroughtherepeatedconnectionofthesameone[1,2 ] .Sinceithasthecharacteristicssuchasnumerousa…  相似文献   
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Objective To study genetic polymorphism of 6 Y chromosomal STR in Hui ethnic group living in Ningxia Hui ethnic autonomous region, in order to evaluate their usefulness in forensic science and enrich the Chinese genetic information resources. Methods We investigated 101 unrelated, healthy, male individuals of Hui ethnic group and studied their allelic frequency distribution and haplotype diversity of 6 Y chromosomal STR. Primer for each loci was labeled with the fluorescent by FAM (blue) or TAMRA(yellow). The data of Hui ethnic group were generated coamplification, GeneScan, genotype, and genetic distribution analysis. Results 31 alleles and 43 phenotype(DYS385) were detected, with the frequencies ranging from 0. 0099- 0. 7129. Out of a total of 101 individuals, 96 showed different haplotypes; 91 were unique; 5 were found 2 times. The haplotype diversity for 6 Y-STR loci was 0. 9990. Conclusion The date obtained can be valuable for individual identification, paternity testing in forensic fields and for population genetics because of 6 Y-STR loci high polymorphism.  相似文献   
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