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植树工程是高速公路环境绿化工程的重要组成部分,然而苗木移栽的成活率直接影响到高速公路整体绿化效果。通过加强施工管理、施工技术等重要环节的控制,渝湛高速公路(粤境段)的环境绿化已取得良好效果,各种苗木的成活率均达95%以上。根据渝湛高速公路(粤境段)的种植土壤及气候条件,结合绿化植树施工经验总结了几点提高成活率的重要控制环节。 相似文献
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Objective To investigate the properties of proliferation and differentiation of neonatal rat retinal progenitor cells (RPCs) in vitro. Methods RPCs were isolated from neonatal SD rats neural retina and cultured in DMEM/F12 + N2 with EGF and bFGF (suspension medium ) or 10 % FBS without EGF and bFGF ( differentiation medium). The cells grew as suspended spheres or adherent monolayers, depending on different culture conditions. The neural stem cells or retinal progenitors, neurons, astrocytes, retinal ganglion cells, rod photoreceptors and the proliferating cells were evaluated with immunofluorescence analysis by Nestin or Pax6, Map2, GFAP, Thy-1, Rhodopsin and BrdU antibodies respectively. Results RPCs could propagate and differentiate in suspension or differentiation medium and express the markers of Nestin (92.86%) or Pax6 (86.75%), Map2 (38.54%), GFAP (20.93%), Thy-1 (27. 66%) and Rhodopsin(13. 33%)in suspension medium; however, Nestin (60.27%), Pax6 (52%), Map2 (34.94%), GFAP (38.17%), Thy-1 (30.84%) and Rhodopsin (34.67%) in differentiation medium. 96.4 % of the population in the neurospheres was BrdU-positive cells. The cells could spontaneously adherent forming some subspheres and retinal specific cell types. Conclusion Neonatal rat RPCs possess the high degree of proliferation and can differentiate into neurons, astrocytes, retinal ganglion cells and rod photoreceptors in vitro. There are different proportions for RPCs to differentiate into specific cell types. 相似文献
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植树工程是高速公路环境绿化工程的重要组成部分,然而苗木移栽的成活率直接影响到高速公路整体绿化效果.通过加强施工管理、施工技术等重要环节的控制,渝湛高速公路(粤境段)的环境绿化已取得良好效果,各种苗木的成活率均达95%以上.根据渝湛高速公路(粤境段)的种植土壤及气候条件,结合绿化植树施工经验总结了几点提高成活率的重要控制环节. 相似文献
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野生狗牙根栽培利用试验研究 总被引:2,自引:0,他引:2
开发利用适宜的野生植物资源应用于高速公路绿化,具有投资小,见效快和护坡性能强的特点,结合高速公路绿化的特点,对当地的野生狗牙根进行了植物学性状和生物学特性的定位观察记载,在对栽培技术进行了研究探讨后,大面积应用于高速公路边坡绿化获国道获成功,实践证明,野生狗牙根在我国南北类似地区的高等级公路绿化中,是一个宝贵的种质资源。 相似文献