Screening of the effective cellulose-degradable strain and its application in the production of cellulose bioethanol

Strains from the cellulose-containing environment were collected. Primary screening(by filter-paper Hutchison solid culture medium and sodium carboxymethylcellulose solid culture medium) and reelection(by filter-paper inorganic salt culture medium and sodium carboxymethylcellulosc Congo red coltnre medium) indicated that five strains obtained were best suited for high performance cellulose degradation. Determination of sodium carboxymethylcellulose activity(CMCA) and filter paper activity(FPA) was accomplished for each of the five. The strongest of the five in CMCA and FPA was applied to the production of cellulose bioethanol by separate hydrolysis and fermentation(SHF) and simultaneous saccharification and fermentation(SSF) respectively.     

科玛嘉培养基与沙氏培养基分离酵母菌的比较

应用科玛嘉显色培养基对１２０份妇科门诊病人分泌物标本进行念珠菌的分离培养。与常用的沙氏培养基比较。方法用新环分别将标本接种于两种培养基上，３７℃培养，２４ｈ和４８ｈ各观察一次，并记录茵落生长时间，大小及颜色。     

多方搭桥西部学子步入航海殿堂——西部首家海员培养基地落户延安职业技术学院

6月26日，延安市政府举行海员培养基地建设新闻发布会暨捐赠签约仪式，今年延安职业技术学院将迎来航海类专业的第一批学生。这标志着西部内陆地区首家海员培养基地正式落成，西部航海事业将揭开崭新的一页，西部地区的莘莘学子将从这里走向蓝色的大海，走向蓝色的未来。     

基因重组人IL-6工程菌发酵培养的实验研究

目的　优化基因重组人IL 6 (rhIL 6 )工程菌的发酵和表达条件。方法　选择五种不同的细菌培养基研究rhIL 6工程菌的发酵培养和诱导表达靶蛋白的最佳时间 ,并监测重组质粒在工程菌中的遗传稳定性。结果　 1 .5×LB培养基收获的生物菌量最大 ,rhIL 6在工程菌中表达率最高 ;42℃诱导 5hrhIL 6表达量最大 ;所构建的重组质粒在工程菌DH5a中传代稳定 ,未见质粒丢失 ,靶蛋白表达未受影响。结论　提示培养基的成分对构建的工程菌生长影响较大 ,应对发酵培养基及诱导表达条件进行优化。     

抓住机遇 建设高水平国际海员培养基地

分析STCW公约马尼拉修正案对航海教育和培训的影响,阐述青岛远洋船员职业学院对修正案的应对策略与发展实践,即积极参与公约修正工作、深入开展教育与培训改革、加强内涵与品牌建设、努力加强实训室建设、着力师资队伍打造与整体素质提升。     

宁波大学海运学院——航运人才的培养基地

宁波大学海运学院是交通运输部向国际海事组织（IMO）推荐的中国首批7所高等航海院校之一，也是香港船东会第一届中国海运院校论坛常务委员会副主席单位。学院以航海类专业为主干，以物流管理专业为拓展，依托和发挥综合大学的优势，促进学科交叉和协调发展；以教学为中心，积极开展科学研究和社会服务，     

Identification and culture of neural stem cells isolated from adult rat subventricular zone following fluid percussion brain injury

Objective To analyze proliferation and differentiation of glial fibrillary acid protein (GFAP)- and nestin-positive (GFAP+/nestin+) cells isolated from the subventricular zone following fluid percussion brain injury to determine whether GFAP+/nestin+ cells exhibit characteristics of neural stem cells. Methods Male Sprague-Dawley rats, aged 12 weeks and weighing 200-250 g, were randomly and evenly assigned to normal control group and model group. In the model group, a rat model of fluid percussion brain injury was established. Five days later, subventricular zone tissue was resected from each group and made into single cell suspension. After serum-free neural stem cell medium culture and subsequent serum-induced differentiation, cell type, proliferation and differentiation capacities were determined by immunofluorescence staining and flow cytometry. Results At 3-7 days after fluid percussion brain injury, nestin+/GFAP+ cells in the single cell suspension from the model group significantly outnumbered those from the normal control group (P<0.01). In the model group, an increased number of small neurospheres with smooth cell edge and bulged center formed after primary culture, and were clearly visible with the increase of culture time and medium replacement. After several passages, many clonal spheres were obtained, suggesting strong self-proliferatiing capacity. Neurospheres from the model group differentiated into astrocytes, neurons and oligodendrocytes. Conclusion GFAP+/nestin+ cells isolated from the adult rat subventricular zone after fluid percussion brain injury are thought to be neural stem cells because of their self-renewal and multi-differentiation capacities.     

浙贝母鳞茎愈伤组织的诱导及植株再生

比较了不同的基本培养基、激素以及激素组合对诱导浙贝母愈伤组织和原球茎的影响．浙贝母愈伤组织和原球茎的诱导及分化与培养基、激素组合和外植体的类型有关．在MS 1．0mg／L2，4-D 1．5mg／L NAA 300mg／L水解酪蛋白 0．55％(质量分数)琼脂培养基中，以浙贝母鳞茎作为外植体诱导愈伤组织的结果最好．在MS 1．0mg／L NAA 2．0mg／L ZT 0．55％琼脂培养基中，从浙贝母鳞茎黄褐色愈伤组织上诱导再生植株效果最好．诱导原球茎最好的培养基是MS 1．0mg／L 2，4-D 2．0mg／L KT 0．55％琼脂。     

微生物絮凝剂产生菌的筛选及其培养基成分优化

通过菌种富集、分离、纯化从天然土壤中筛选出三株具有较高絮凝活性的微生物絮凝剂产生菌(MB1-6-5、MB6-6-3、MB6-6-11),并对其中絮凝活性最高的MB6-6-11培养基成分进行了正交实验,找出了最佳培养基成分比例.经优化后,MB6-6-11对高岭土悬浊液的絮凝率可达93.6%.