胆囊癌组织及胆汁中厌氧菌谱的检测

为探讨厌氧菌感染与胆囊组织癌变的相关关系 ,采用微生物自动检测鉴定系统 ,对 2 9例胆囊癌患者的胆囊胆汁及 9例胆囊癌中心组织进行厌氧菌培养及自动检测分析。结果 2 9例胆囊癌胆汁中厌氧菌检出率为 6 5 .5 % ( 1 9/2 9) ,胆囊癌组织检出率为 5 5 .6 % ( 5 /9) ,且均为兼性厌氧。两者厌氧菌株均以产气荚膜梭菌为主。认为胆囊粘膜癌变可能与厌氧菌尤其是产气荚膜梭菌感染有关 ,厌氧菌与需氧菌协同作用 ,长期刺激胆囊粘膜是导致其癌变的主要因素。而利用微生物生长曲线中对数生长期进行培养是提高厌氧菌阳性率的关键。

DETECTION OF ANAEROBIC BACTERIA IN TISSUE AND BILE FROM GALLBLADDER CARCINOMA

In order to find out the relation between infection of anaerobic bacteria gallbladder carcinoma,we used microbial detection and determine system to undergo anaerobic bile culture in 29 patients with gallbladder carcinoma were also detected by this method.The ratio of anaerobic bacteria in bile was 65.5%(19/29) in tissue was 55 6% (5/9) and all with aerobic positive.C perfringers was the main kind of anaerobic bacteria in bile ( 52.4% ) and tissue (80%).It suggests that some relation between gallbladder carcinoma and infection of anaerobic bacteria, especially of C perfringers.Anaerobic and aerobic bacteria stimulating gallbladder mucosa together is the main reason that lead to formation of carcinoma.Cultruring at logarithmic phase can improve the positive rate of anerobic phase can improve the positive rate of anaerobic culture.