| EXPRESSION CLONING OF A PROTECTIVE LEISHMANIA ANTIGEN |
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| 作者姓名： | 郑时春 |
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| 作者单位： | Institute de Pharmacologie Moleculaire et Cellulaire，Valbonne，France and Department of Medicine and Microbiology /Immunology，Univ. of Calif |
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| 摘 要： | ＥＸＰＲＥＳＳＩＯＮＣＬＯＮＩＮＧＯＦＡＰＲＯＴＥＣＴＩＶＥＬＥＩＳＨＭＡＮＩＡＡＮＴＩＧＥＮＥ．Ｍｏｕｇｎｅａｕ，Ｆ．Ａｌｔａｒｓ，Ａ．Ｅ．Ｗａｋｉｌ，ＺｈｅｎｇＳｈｉｃｈｕｎ；Ｔ．Ｃｏｐｐｏｌａ，Ｚ．Ｗａｎｇ，Ｒ．Ｗａｌｄｍａｎｎ，Ｒ．Ｍ．Ｌｏｃ...
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EXPRESSION CLONING OF A PROTECTIVE LEISHMANIA ANTIGEN |
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| E. Mougneau,F. Altars,A. E. Wakil,Zheng Shichun,T. Coppola,Z. Wang,R. Waldmann,R. M. Locksley,N. Glaichenhaus.EXPRESSION CLONING OF A PROTECTIVE LEISHMANIA ANTIGEN[J].Academic Journal of Xi’an Jiaotong University,1995(2). |
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| Authors: | E Mougneau F Altars A E Wakil Zheng Shichun T Coppola Z Wang R Waldmann R M Locksley N Glaichenhaus |
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| Abstract: | Parasite-specific CD8 T cells have been shown to transfer protection against nLeishmania major in susceptible BALB/c mice.An epitope-tagged expression library was used to identify the antigen recognized by a protective CD8 T cells clone. The expression library allowed recombinant proteins made in bacteria to be captured by macrophages for presentation to T cells restricted to major histompatibility complex class n. A conserved 36-kilodalton member of the tryptophanaspartic acid repeat family of proteins was identified that was expressed in both stages of the parasite life cycle. A 24-kilodalton portion of this antigen protected susceptible mice when administered as a vaccine with interleukin-12before injection. |
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| Keywords: | Leishmania major expression cloning protective antigen vaccine CDs4 cell |
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