| 免疫酶染技术用于流行性出血热早期诊断的初步研究 |
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| 引用本文： | 岐天茂,吴文琪,谢蔚玫.免疫酶染技术用于流行性出血热早期诊断的初步研究[J].西安交通大学学报(医学版),1982(4). |
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| 作者姓名： | 岐天茂 吴文琪 谢蔚玫 |
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| 作者单位： | 西安医学院病毒研究室
(岐天茂，吴文琪)，西医附一院传染科(谢蔚玫) |
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| 摘 要： | <正> 长期以来流行性出血热(以下简称出血热)的早期诊断主要是根据临床症状及流行病学资料,这是因为病毒分离未能成功,实验室缺乏有效敏感方法,从而给防治工作带来一定困难。近年来有人报导用免疫酶染技术(简称酶染)检查病理组织及培养细胞中的病毒颗粒,启示我们使用直接法对出血热早期患者外周血白细胞进行研究。现将1981年11月至1982年元月我省流行性出血热流行季节新采取的145伤外周血白细胞的酶染结果报告如下。
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The Early Diagnosis of Epidemic Hemorrhagic Fever With Immunoenzymatic Technique |
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| Abstract: | This paper recommends the early diagnosis of epidemic hemorrhagicfever with immunoenzymatic direct staining (IDS) to study the patient'speripheral white blood cells. The samples of blood smears from 63 patientswere examined under the microscope with this metbod. In 54 patients, the results were positive (85. 71%). We systemically observed 10 blood sam-ples from the patients in different courses of the disease, and found thatduring the period of recovery the results were negative. This method wasalso used to observe the blood smears from both 22 patients infected withother viruses and 20 healthy persons, and a negative result was obtained.It was demonstrated that IDS had stronger specificity. The advantages ofthis method are that blood samples are easily taken, carried and preserved,and that the experimental method is simple. Thus, IDS is of practical signifi-cance in preventing and treating the disease. |
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