| 使用WEHI细胞和MTT试验检查单核细胞的细胞毒性 |
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| 引用本文： | 王一理,司履生.使用WEHI细胞和MTT试验检查单核细胞的细胞毒性[J].西安交通大学学报(医学版),1992(3). |
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| 作者姓名： | 王一理 司履生 |
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| 作者单位： | 西安医科大学免疫病理研究室，西安医科大学免疫病理研究室 西安 710061，西安 710061 |
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| 摘 要： | 以TNF-α敏感细胞株WEHI克隆13为靶细胞,用MTT比色法代替传统的~(51)Cr释放试验检测单核细胞的细胞毒性,本法有更大的优点,快速、敏感,所需效应细胞量少,更适于临床应用,而且无同位素污染。
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| 关 键 词： | MTT 单核细胞 细胞毒测定 |
USING WEHI CELL LINE AS A TARGET AND MTT COLORIMETRIC TECHNIQUE FOR MONOCYTES CYTOTOXIC ASSAY |
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| Wang Yili,Si Lusheng.USING WEHI CELL LINE AS A TARGET AND MTT COLORIMETRIC TECHNIQUE FOR MONOCYTES CYTOTOXIC ASSAY[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1992(3). |
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| Authors: | Wang Yili Si Lusheng |
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| Institution: | Immunopathology Lab. Department of pathology |
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| Abstract: | We used WEHI clone—13 cell line as target and MTT colorimetric technique to evaluate monocyte cytotoxicity, this method is superior to the traditional~(51) Cr release technique. It is more sensitive,more rapid and less effectors needed, as well as no isotope contamination, and it is mose suitable in clinic. |
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| Keywords: | MTT monocyte cytotoxic assay |
| 本文献已被 CNKI 等数据库收录！ |
