| THE CONSTRUCTION AND PRELIMINARY APPRAISEMENT OF HSV-2 gD GENE DNA VACCINE |
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| 作者姓名： | 王军阳 范桂香 胜利 袁育康 |
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| 作者单位： | Department of Immunology，Medical School of Xi'an Jiaotong University，Department of Immunology，Medical School of Xi'an Jiaotong University，Department of Immunology，Medical School of Xi'an Jiaotong University，Department of Immunology，Medical School of Xi'an Jiaotong University Xi'an 710061，China，Xi'an 710061，China，Xi'an 710061，China，Xi'an 710061，China |
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| 摘 要： | Ｈｅｒｐｅｓｓｉｍｐｌｅｘｖｉｒｕｓｔｙｐｅ 2 (ＨＳＶ 2 ) ,ｏｎｅｋｉｎｄｏｆｄｏｕｂｌｅｓｔｒａｎｄｓＤＮＡｖｉｒｕｓ ,ｃａｎｃａｕｓｅｓｅｒｉｏｕｓｗｏｒｌｄｗｉｌｄｉｎｆｅｃｔｉｏｎｓ.Ｈｅｒｐｅｓｓｉｍｐｌｅｘｖｉｒｕｓｇｅｎｏｍ ｉｃＤＮＡｃｏｄｅｓｆｏｒａｐｐｒｏｘｉｍａｔｅｌｙ 80 ｐｒｏｔｅｉｎｓ .Ｍｏｓｔｏｒａｌｌａｒｅｅｘｐｒｅｓｓｅｄｄｕｒｉｎｇｌｙｔｉｃｉｎｆｅｃｔｉｏｎ ,ｍａｎｙａｒｅｆｏｕｎｄｉｎｉｎｔａｃｔｖｉｒｉｏｎｓａｎｄａｔｌｅａｓｔ 11ｈａｖｅｂｅｅｎｉｄｅｎｔ…
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THE CONSTRUCTION AND PRELIMINARY APPRAISEMENT OF HSV-2gD GENE DNA VACCINE |
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| Wang Junyang,Fan Guixiang,Sheng li,Yuan Yukang.THE CONSTRUCTION AND PRELIMINARY APPRAISEMENT OF HSV-2 gD GENE DNA VACCINE[J].Academic Journal of Xi’an Jiaotong University,2002,14(2):163-165. |
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| Authors: | Wang Junyang Fan Guixiang Sheng li Yuan Yukang |
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| Institution: | Department of Immunology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. |
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| Abstract: | Objective To prevent infection from herpes simplex virus type 2(HSV-2),and make the foundation for the construction of multi-valent DNA vaccine. Methods The complete DNA sequence,which encoded the amino acid sequence of the viral glycoprotein D(gD),was obtained from the HSV-2 genome by polymerase chain reaction(PCR).The fragment was inserted into the lower stream of Cytomegalovirus(CMV) promoter in the eukaryotic expression plasmid pcDNA3.1(+), then immunized mice by bilateral intramuscular injection into the rear legs with this recombinant plasmid and tested the specific antibodies against glycoprotein D by ELISA. Results Animal experiment have demonstrated that the recombinant plasmid(pcDNA-gD2)inoculated into mice could induce the production of specific antibodies against glycoprotein D. Conclusion The eukaryotic plasmid pcDNA-gD2 constructed by us could correctly express gD gene and induce the production of specific antibodies. |
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| Keywords: | HSV-2 Glycoprotein D DNA vaccine PCR |
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