| DESIGN OF ANTI-CD3 ScFv-B7.1 FUSION MOLECULE AND PREDICTION OF ITS BIOLOGICAL CHARACTERISTICS |
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| 作者姓名： | 杨章民 司履生 王一理 来宝长 |
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| 作者单位： | Institute for Cancer Research，School of Life Science & Technology，Institute for Cancer Research，School of Life Science & Technology，Institute for Cancer Research，School of Life Science & Technology，Institute for Cancer Research，School of Life Science & Technology Xi'an Jiaotong University，Xi'an 710061，China，Xi'an Jiaotong University，Xi'an 710061，China，Xi'an Jiaotong University，Xi'an 710061，China，Xi'an Jiaotong University，Xi'an 710061，China |
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| 基金项目： | ThisworkwassupportedbytheScientificResearchFoundationofHealthMinistryofChina(No .98 1 2 32 ) |
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| 摘 要： | Ｇｅｎｅｔｉｃａｌｌｙｅｎｇｉｎｅｅｒｅｄｂｉｆｕｎｃｔｉｏｎａｌ ｐｒｏｔｅｉｎｓｈａｖｅｓｈｏｗｎ ｐｒｏｍｉｓｉｎｇ ｐｒｏｓｐｅｃｔｓｉｎｔｕｍｏｒｃｌｉｎｉｃａｌａｄｍｉｎｉｓｔｒａｔｉｏｎａｎｄｂａｓｉｃｒｅｓｅａｒｃｈ .Ａｎｔｉ ＣＤ3ＳｃＦｖ(ａｎｔｉ ＣＤ3Ｓｉｎｇｌｅ ｃｈａｉｎｆｒａｇｍｅｎｔｏｆｖａｒｉａｂｌｅｒｅ ｇｉｏｎ)ｉｓａｋｉｎｄｏｆｒｅｃｏｍｂｉｎａｎｔｅｎｇｉｎｅｅｒｅｄａｎｔｉｂｏｄｙｄｅｒｉｖｅｄｆｒｏｍｍｕｒｉｎｅＯＫＴ3ｈｙｂｒｉｄｏｍａ .Ａｓａｐｏｔｅｎｔｍｉ…
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DESIGN OF ANTI-CD3 ScFv-B7.1 FUSION MOLECULE AND PREDICTION OF ITS BIOLOGICAL CHARACTERISTICS |
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| Institution: | Institute for Cancer Research, School of Life Science & Technology, Xi'an Jiaotong University, Xi'an 710061,China. |
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| Abstract: | Objective To design and construct the eukaryotic expression vector which expresses Anti-CD3 ScFv-B7.1 fusion molecules and predict the biological characteristics, the rationality and feasibility of the spacer. Methods To analyze the flexibility, Hoop & Woods hydrophilicity and the epitope of Anti-CD3 ScFv-B7.1 fusion molecule at secondary structure level by computer simulation utilizing the GoldKey software. Results By comparing with Anti-CD3 ScFv and B7.1 respectively, it shows that Anti-CD3 ScFv-B7.1 fusion molecules can form correct secondary structure with the linking of the spacer, the fusion does not change the original hydrophilicity and epitopes of both Anti-CD3 ScFv and B7.1, no new epitopes emerge; The spacer is flexible and shows low antigenicity. Conclusion The design of Anti-CD3 ScFv-B7.1 fusion molecule are rational and feasible, the expressed fusion protein could retain the maximum biological activity and the function of both Anti-CD3 ScFv and B7.1. |
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| Keywords: | anti-CD3 ScFv B7 1 fusion gene computer simulation |
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