| DESIGN OF ANTI-CD3 ScFv-B7.1 FUSION MOLECULE AND PREDICTION OF ITS BIOLOGICAL CHARACTERISTICS |
| |
| 引用本文： | 杨章民,司履生,王一理,来宝长. DESIGN OF ANTI-CD3 ScFv-B7.1 FUSION MOLECULE AND PREDICTION OF ITS BIOLOGICAL CHARACTERISTICS[J]. 西安交通大学学报(英文版), 2002, 14(2): 117-120 |
| |
| 作者姓名： | 杨章民 司履生 王一理 来宝长 |
| |
| 作者单位： | Institute for Cancer Research，School of Life Science & Technology，Institute for Cancer Research，School of Life Science & Technology，Institute for Cancer Research，School of Life Science & Technology，Institute for Cancer Research，School of Life Science & Technology Xi'an Jiaotong University，Xi'an 710061，China，Xi'an Jiaotong University，Xi'an 710061，China，Xi'an Jiaotong University，Xi'an 710061，China，Xi'an Jiaotong University，Xi'an 710061，China |
| |
| 基金项目： | ThisworkwassupportedbytheScientificResearchFoundationofHealthMinistryofChina(No .98 1 2 32 ) |
| |
| 摘 要： | Ｇｅｎｅｔｉｃａｌｌｙｅｎｇｉｎｅｅｒｅｄｂｉｆｕｎｃｔｉｏｎａｌ ｐｒｏｔｅｉｎｓｈａｖｅｓｈｏｗｎ ｐｒｏｍｉｓｉｎｇ ｐｒｏｓｐｅｃｔｓｉｎｔｕｍｏｒｃｌｉｎｉｃａｌａｄｍｉｎｉｓｔｒａｔｉｏｎａｎｄｂａｓｉｃｒｅｓｅａｒｃｈ .Ａｎｔｉ ＣＤ3ＳｃＦｖ(ａｎｔｉ ＣＤ3Ｓｉｎｇｌｅ ｃｈａｉｎｆｒａｇｍｅｎｔｏｆｖａｒｉａｂｌｅｒｅ ｇｉｏｎ)ｉｓａｋｉｎｄｏｆｒｅｃｏｍｂｉｎａｎｔｅｎｇｉｎｅｅｒｅｄａｎｔｉｂｏｄｙｄｅｒｉｖｅｄｆｒｏｍｍｕｒｉｎｅＯＫＴ3ｈｙｂｒｉｄｏｍａ .Ａｓａｐｏｔｅｎｔｍｉ…
|
DESIGN OF ANTI-CD3 ScFv-B7.1 FUSION MOLECULE AND PREDICTION OF ITS BIOLOGICAL CHARACTERISTICS |
| |
| Affiliation: | Institute for Cancer Research, School of Life Science & Technology, Xi'an Jiaotong University, Xi'an 710061,China. |
| |
| Abstract: | Objective To design and construct the eukaryotic expression vector which expresses Anti-CD3 ScFv-B7.1 fusion molecules and predict the biological characteristics, the rationality and feasibility of the spacer. Methods To analyze the flexibility, Hoop & Woods hydrophilicity and the epitope of Anti-CD3 ScFv-B7.1 fusion molecule at secondary structure level by computer simulation utilizing the GoldKey software. Results By comparing with Anti-CD3 ScFv and B7.1 respectively, it shows that Anti-CD3 ScFv-B7.1 fusion molecules can form correct secondary structure with the linking of the spacer, the fusion does not change the original hydrophilicity and epitopes of both Anti-CD3 ScFv and B7.1, no new epitopes emerge; The spacer is flexible and shows low antigenicity. Conclusion The design of Anti-CD3 ScFv-B7.1 fusion molecule are rational and feasible, the expressed fusion protein could retain the maximum biological activity and the function of both Anti-CD3 ScFv and B7.1. |
| |
| Keywords: | anti-CD3 ScFv B7.1 fusion gene computer simulation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
