DISTRIBUTION OF PARVALBUMIN, CALBINDIN-D28 AND CALRETININ IMMUNOREACTIVE NEURONS AND FIBERS IN THE MONKEY BASAL GANGLIA

Ｐａｒｖａｌｂｕｍｉｎ (ＰＶ ) ,Ｃａｌｂｉｎｄｉｎ Ｄ2 8(ＣＢ)ａｎｄＣａｌｒｅｔｉｎｉｎ (ＣＲ)ａｒｅｍｅｍｂｅｒｓｏｆｔｈｅＥＦ ｈａｎｄｆａｍｉｌｙｏｆｃａｌｃｉｕｍ ｂｉｎｄｉｎｇ ｐｒｏｔｅｉｎｓ (ＣａＢＰｓ) .Ｔｈｅｓｅｔｈｒｅｅｐｒｏｔｅｉｎｓ,ｗｈｉｃｈｈａｖｅａｌｏｗｍｏｌｅｃｕｌａｒｗｅｉｇｈｔａｎｄａｈｉｇｈａｆｆｉｎｉｔｙｆｏｒｃａｌｃｉｕｍｉｏｎｓ ,ａｃｔ ｐｒｉｎｃｉｐａｌｌｙａｓｉｎｔｒａｃｅｌｌｕｌａｒｃａｌｃｉｕｍｉｏｎｓｂｕｆｆｅｒｓ .Ｔｈｅｙａｌｓｏｐｒｏｂａｂｌｙ…

DISTRIBUTION OF PARVALBUMIN, CALBINDIN-D28 AND CALRETININ IMMUNOREACTIVE NEURO NS AND FIBERS IN THE MONKEY BASAL GANGLIA

Objective To investigate the cellular localization of parvalbumin (PV), calbindin-D28k (CB) and calretinin (CR) in the monkey basal ganglia.Methods Immunocytochemical technique was used to detect PV,CB and CR immunoreactivity in the basal ganglia. Results In the striatum, CB labeled medium-sized spiny projection neurons whereas PV and CR marked two separate classes of aspiny interneurons. The striatal matrix compartment was markedly enriched with CB while striatal patches displayed a CR-rich neuropil. In the pallidum, virtually all neurons contained PV but none express CB. CR occured only in a small subpopulation of large and small pallidal neurons. In the subthalamic nucleus, there existed a multitude of PV-positive cells and fibers but the number of CR and CB-positive neuronal elements was small. In the substantia nigra / ventral tegmental area complex, CB and CR occured principally in dopaminergic neurons of the dorsal tier of the pars compacta and in those of the ventral tegmental area. PV was strickly confined to the GABAergic neurons of the pars reticular and lateralis. CB-rich fibers abounded in the pars reticular and lateralis, while CR-positive axons were confined to the pars compacta. Conclusion CB and PV were distributed according to a strikingly complementary pattern in primate basal ganglia, and the use of CB and PV immunocytochemistry may be considered as an excellent tool to define distinct chemoarchitectonic and functional domains within the complex organization of the basal ganglia. CR was less ubiquitous but occured in small basal ganglia components where it labeled distinct subsets of neurons. Such highly specific patterns of distribution indicate that CB, PV and CR may work in synery within primate basal ganglia.