甲状腺癌中血管内皮生长因子、成纤维细胞生长因子3的表达及意义

目的探讨血管内皮生长因子(VEGF)及成纤维细胞生长因子3(FGF3)在甲状腺良、恶性病变组织中的表达及其表达与甲状腺癌临床病理特征的关系。方法采用免疫组织化学S-P和SABC法检测61例甲状腺癌、43例甲状腺良性病变和19例癌旁组织标本的VEGF及FGF3的表达。结果甲状腺癌、甲状腺良性病变和癌旁组织VEGF阳性表达率分别为86.9%、16.3%、10.5%,FGF3阳性表达率分别为60.7%、7.0%及5.3%的,甲状腺癌与甲状腺良性病变和癌旁组织比较,VEGF及FGF3的阳性表达率均有显著性差异(P<0.05)。VEGF在甲状腺癌T3/T4期组、预后不良组及复发组的强阳性表达率均较对照组高(P<0.05),VEGF染色分数与甲状腺癌预后指数呈正相关(r=0.323,P=0.011);FGF3的表达与甲状腺癌组织学类型、肿瘤分期等临床病理参数无相关(P>0.05),但在VEGF阳性的甲状腺癌中有更高的表达(P<0.05)。结论VEGF及FGF3的过表达可作为协助甲状腺癌诊断的一个指标,两者在甲状腺癌中有协同表达;VEGF是甲状腺癌的预后预示因子。

Significance of the expression of VEGF and FGF3 in thyroid carcinoma

Objective To investigate the expression of VEGF and FGF3 gene product in benign and malignant thyroid diseases and explore the relationship between their expression and the clinicopathological features of thyroid carcinoma.Methods The expression of VEGF and FGF3 were determined by immunohistochemistrical staining technique(S-P and SABC method) in 61 cases of thyroid carcinomas(containing 43 papillary,14 follicular and 4 anaplastic carcinomas),43 cases of hyperplastic lesions(including 31 goiters and 12 thyroid adenomas)and 19 adjacent tissue that had been fixed in formalin and embedded in paraffin.Results The rate of positive expression of VEGF was 86.9%, 16.3% and 10.5% while that of FGF3 was 60.7%,7.0% and 5.3% in tumor tissue,hyperplastic lesions and adjacent tissue,respectively.The expression of VEGF and FGF3 was located at cytoplasm and extracellular matrix;the positive expression and strong expression of VEGF and FGF3 were elevated significantly in tumor tissue compared to hyperplastic lesions and adjacent tissue;the proportion of over-expression of VEGF was statistically higher in patients at T3/T4 stage,poorprognostic and recurrence patients compared to control cases(P<0.05);there was a positive correlation between the staining score of VEGF and the prognosis score of TC(r=0.323,P=0.011).On the other hand,There was no significant difference between the expression of FGF3 and all of the clinicopathological features of thyroid cancer.Conclusion There is a positive relationship between the expression of VEGF and that of FGF3 in TC;the overexpression of VEGF and FGF3 is a auxiliary diagnosis marker for thyroid carcinoma.VEGF can be used as a predictor for prognosis of TC;the overexpression of VEGF in tumor tissue can predict lower differentiation,greater aggressive course and worse prognosis.