Na~+通道阻断剂对脑缺血海马锥体细胞损伤的保护作用

实验为观察在脑一过性缺血条件下，Ｎａ＋ 通道阻断剂对海马ＣＡ１ 区锥体细胞是否具有保护作用，对９ 月龄Ｗｉｓｔａｒ大鼠进行实验。通过脑室内单独注射利多卡因、利多卡因和呋喃苯胺酸复合应用，用Ｈ Ｅ染色方法通过计算机对海马ＣＡ１ 区神经元计数。结果发现，单独注射２％ 利多卡因５μｌ，对海马ＣＡ１ 区锥体细胞无保护作用；２％ 利多卡因和２％ 呋喃苯胺酸各２５μｌ联合应用，与对照组相比，具有显著性差异（ Ｐ＜ ０．０１）。实验结果提示，Ｎａ＋ 通道阻断剂联合应用，可能是预防脑缺血引起海马ＣＡ１ 区神经细胞损伤的重要途径之一

THE PROTECTIVE EFFECT OF Na+ CHANNEL BLOCKERS AGAINST CELL DAMAGE CASUSED BY ISCHEMIA

In order to observe it blockers of sodium channel obsesses the neuroprotective effect on hippocampal CA 1 pyramidal cell under the condition of transient brain ischemia,the present experiment used 24 male Wistar rats aged 9 months and divided them into four groups.Lidocaine and/or furosemide were injected introcerebroventicularlly(I.C.V).Stained with H E and accounted the CA 1 pyramidal cell numbers by computer in each group suggested following findings:Although 5μl of 2% lidocaine was injected I.C.V ,the results indicated lidocaine didn't have any blockade to pyramidal cell injuries in hippocampal CA 1 area(P<0.05).In the group medicated with 2.5μl of both 2% lidocaine and 2% furosemide, the results showed that the combined approach had a blockade to injuries of pyramidal cells compared with control group (P<0.01).The present experiment indicates that the combined blockade of lidocaine and furosemide injected I.C.V. to Na + channel can prevent the injuries from hippocampal neurons owing to ischemia.