大鼠混合反流模型中COX-2、PCNA、Cyclin D_1的表达

目的探讨环氧合酶-2(COX-2)、增殖细胞核抗原(PCNA)及细胞周期蛋白D1(Cyclin D1)这3项指标在反流性食管炎的发生、发展及癌变过程中的变化情况。方法健康SD大鼠54只,随机分为2组:反流模型组46只,正常对照组8只。分别观察反流模型组术后5、17、28、40周,正常对照组40周时食管黏膜的病理变化,检测COX-2、PCNA、Cyclin D1的表达情况。结果在反流性食管炎的发展过程中,COX-2、PCNA、Cyclin D1从正常→反流性食管炎(RE)→Barrett食管(BE)→食管腺癌(EAC)的阳性表达率分别为0.0%、42.1%、73.7%、100.0%;0.0%、42.1%、63.2%、100.0%;12.5%、42.1%、63.2%、100.0%。COX-2、PCNA、Cyclin D1的表达程度逐渐增强。RE、BE、EAC的表达明显高于正常对照(P<0.05),RE与BE、EAC间有显著性差异(P<0.05),BE和EAC间无统计学差异可能是EAC的例数较少。结论在混合反流造成黏膜损伤形成RE的同时,COX-2、PCNA和Cyclin D1的表达增强,并随病程的发展逐渐增强。说明COX-2、PCNA和Cyclin D1基因的高表达参与了从RE→BE→EAC的发展过程,是BE、EAC发生、发展的早期分子事件。

The expression of COX-2, PCNA and Cyclin D1 in the model of reflux esophagitis

Objective To investigate the expression of COX-2, PCNA and Cyclin D1 in the progress of reflux esophagitis. Methods A total of 54 SD rats were divided into two groups randomly model group were treated with esophagoduodenostomy; control group were normals. The lesions of esophageal mucosa were observed in the 5^th, 17^th, 28^th, 40^th week in model group and 40^th week in control group, respectively. The changes of COX-2, PCNA and Cyclin D1 were evaluated by immunoperoxidase staining in the progress of reflux esophagitis. Results The positive rates of COX-2, PCNA and Cyclin D1 were 0.0%, 0.0% and 12.5% in normal respectively, and were 42.1%, 42.1% and 42.1% in RE respectively, 73.7% , 63.2% and63.2% in BE, and100. 0% ,100. 0% and 100. 0% in EAC, respectively. The expression of COX-2, PCNA and Cyclin D1 increased gradually with the development of reflux esophagitis, and significant difference was found between RE, BE and EAC, no difference was found between BE and EAC. Conclusion In the progress of RE, COX-2, PCNA and Cyclin D1 increased gradually.