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| 非洛地平对氧化损伤的人脐静脉内皮细胞的保护作用 | |
| 引用本文： | 祁杰,盛婴,郑见宝,袁祖贻.非洛地平对氧化损伤的人脐静脉内皮细胞的保护作用[J].西安交通大学学报(医学版),2010,31(4). |
| 作者姓名： | 祁杰 盛婴 郑见宝 袁祖贻 |
| 作者单位： | 1. 西安交通大学医学院第一附属医院,心血管内科,陕西西安,710061 2. 西安交通大学医学院第一附属医院,干部病房,陕西西安,710061 3. 西安交通大学医学院第一附属医院,普外科,陕西西安,710061 |
| 摘 要： | 目的 研究非洛地平(felodipine)对氧化型低密度脂蛋白(ox-LDL)损伤的人脐静脉内皮细胞(HUVECs)活性氧(ROS)及细胞间粘附分子-1(ICAM-1)、血管细胞粘附分子-1(VCAM-1)mRNA表达的影响,探讨其独立于降血压外的抗动脉粥样硬化的作用机制.方法 筛选ox-LDL与HUVECs细胞孵育最适的干预浓度梯度与时间,然后与不同浓度的非洛地平(0.1、1、10μmol/L)共同孵育24h,流式细胞仪测定细胞内ROS,real time-PCR检测细胞ICAM-1、VCAM-1mRNA的表达.结果 ox-LDL对HUVECs内ROS的产生呈浓度依赖效应,随着刺激浓度的升高,ROS产生增多,25mg/L ox-LDL作用最为显著(P<0.01),非洛地平可抑制ox-LDL诱导的HUVECs ROS(P<0.05)的产生;随ox-LDL刺激浓度的增加,ICAM-1、VCAM-1 mRNA表达逐渐上调,当浓度达到25mg/L时,作用最为显著(P<0.05),非洛地平可下调ICAM-1、VCAM-1 mRNA的表达(P<0.05).结论 非洛地平可抑制ox-LDL诱导的HUVECs ROS的产生以及下调ICAM-1、VCAM-1 mRNA表达,发挥抗氧化抗炎的内皮保护功能. |
| 关 键 词： | 非洛地平 氧化型低密度脂蛋白 氧化应激 动脉粥样硬化 人脐静脉内皮细胞 细胞间粘附分子-1 血管细胞粘附分子-1 |
Protective effect of felodipine on human umbilical vein endothelial cells injured by oxidized low-density lipoprotein |
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| QI Jie,SHENG Ying,ZHENG Jian-bao,YUAN Zu-yi.Protective effect of felodipine on human umbilical vein endothelial cells injured by oxidized low-density lipoprotein[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2010,31(4). | |
| Authors: | QI Jie SHENG Ying ZHENG Jian-bao YUAN Zu-yi |
| Institution: | QI Jie1,SHENG Ying2,ZHENG Jian-bao3,YUAN Zu-yi1(1. Department of Cardiovasology,2. Department of Cadres' Wards,3. Department of General Surgery,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China) |
| Abstract: | Objective To investigate the protective effect of felodipine on reactive oxygen species (ROS) generation,mRNA level of inflammatory factors such as intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1),in human umbilical vein endothelial cells (HUVECs) injured by oxidized low-density lipoprotein (ox-LDL) so as to explore felodipine's anti-atherosclerosis mechanism independent of its anti-hypertensive effect. Methods Isolated HUVECs were treated with ox-LDL at different co... |
| Keywords: | felodipine oxidized low-density lipoprotein (ox-LDL) oxidative stress atherosclerosis human umbilical vein endothelial cell intercellular adhesion molecule-1 (ICAM-1) vascular cell adhesion molecule-1 (VCAM-1) |
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