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| ~(125)Ⅰ—联结法标记人超氧化物歧化酶 | |
| 引用本文： | 王德全,赵利军,贾鹏翔. ~(125)Ⅰ—联结法标记人超氧化物歧化酶[J]. 西安交通大学学报(医学版), 1989, 0(2) |
| 作者姓名： | 王德全 赵利军 贾鹏翔 |
| 作者单位： | 西安医科大学核医学研究室(王德全，赵利军)，西安医科大学核医学研究室(贾鹏翔) |
| 摘 要： | ~(125)I-人超氧化物歧化酶是采用联结法进行标记,先标记羟苯丙酸酯,(Bolton Hunter Reagent),再将~(125)I-B.H.R 与SOD 相联。~(125)I-B.H.R 标记率为95%,放射性比度可达0.376 mci/μg,稳定性在两个月以上。~(125)I-SOD 联结标记率为50%,放射性比度为3.04μci/μg,用醋酸纤维膜和聚丙烯酰胺凝胶电泳后进行放射性层析扫描,放化纯在95%以上,标记不影响该酶的生物活性和免疫活性,冷冻干燥,-20℃保存两个月不失去免疫活性,放化纯仍在95%以上。 |
| 关 键 词： | 超氧化物歧化酶 放射性碘标 联结法 |
THE LABEL OF SUPEROXIDE DISMUTASE WITH ~(125)I USING A CONJUNCTION METHOD |
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| Wang Dequan,et al. THE LABEL OF SUPEROXIDE DISMUTASE WITH ~(125)I USING A CONJUNCTION METHOD[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 1989, 0(2) | |
| Authors: | Wang Dequan et al |
| Affiliation: | Research Laboratory of Nuclear Medicine |
| Abstract: | Superoxide Dismutase(SOD)from human wasradi(?)iodinated with conjuction method:first the BoltonHunter reagent with ~(125)I using a chloramine T method,se-con ~(125)I-BHR was conjunction with SOD,Iodinatedeffeiciency of ~(125)I-Boltton Hunter reagent was 95%,itsspecific activity was 0.376 mCi/μg and it can be stableover two months.~(125)I-SOD was purified by columnchromatography.The conjuncted efficiency was 50%.Itsspecific activity was 3.04μCi/μg.~(125)I-SOD showed agood bioactivity and immunoactivity.Freezing anddrying.~(125)I-SOD was stored at -20℃.It can be sta-ble over two months. |
| Keywords: | superoxide dismutase radioidination conjunction method |
| 本文献已被 CNKI 等数据库收录！ | |