白血病瘤苗对小鼠细胞毒性T淋巴细胞作用的研究

目的　探讨自制的一种白血病疫苗对C57BL/ 6小鼠细胞毒性T淋巴细胞 (CTL)杀伤功能的影响。方法　建立白血病荷瘤小鼠模型 ,用自制的 3种不同的白血病疫苗进行预防或主动免疫治疗 ,用MTT比色法检测预防或治疗 2周、4周后小鼠CTL杀伤活性 ,并与对照组相比较。结果　①随着白血病细胞在小鼠体内的生长 ,小鼠的CTL杀伤功能受到严重抑制。②灭活肿瘤细胞 +IFA +细胞因子 (rGM CSF +rIL 2 +rIL 6)的肿瘤疫苗在提高小鼠CTL的杀伤功能方面 ,优于灭活肿瘤细胞 +IFA肿瘤疫苗 ,而仅含灭活肿瘤细胞的疫苗作用不明显。结论　灭活肿瘤细胞 +IFA +细胞因子 (rGM CSF +rIL 2 +rIL 6)的肿瘤疫苗可以激活以CTL为代表的特异性细胞免疫功能 ,此种疫苗在血液恶性肿瘤的特异性主动免疫治疗中很有潜力

Study on the cytotoxic T lymphocytes of mice treated with leukemia vaccine

Objective To study the cytotoxicity of cytotoxic T lymphocytes (CTL) derived from C 57BL/6 mice treated with the leukemia vaccine prepared by us. Methods We established leukemia burden mice and prepared three types of leukemia vaccine, which were administered on the mice respectively. The MTT colorimetric method was adopted 2 and 4 weeks later to measure the cytotoxicity of CTL derived from the mice which accepted immunotherapy or prevention. Then we compared it with that of control group. Results ① With the growth of leukemia cells in the mice, the cytotoxicity of CTL was severely depressed. ② The tumor vaccine prepared from inactivated tumor cells, IFA and cytokines (rGM-CSF, rIL-2 and rIL-6) promoted the cellular immunity of tumor burden mice more efficiently than the tumor vaccine made from either inactivated tumor cells, IFA or inactive tumor cells themselves alone. Conclusion The tumor vaccine prepared with inactive tumor cells, IFA and cytokines (rGM-CSF, rIL-2 and rIL-6) can activate the specific cellular immunity. Therefore, the tumor vaccine provides a promising future in the active specific immunotherapy against hematopoietic tumors.