| β淀粉样肽1～42单克隆抗体的制备 |
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| 引用本文： | 董炜疆,胡海涛,冯改丰,杨广笑,王全颖.β淀粉样肽1～42单克隆抗体的制备[J].西安交通大学学报(医学版),2004,25(5):517-519. |
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| 作者姓名： | 董炜疆 胡海涛 冯改丰 杨广笑 王全颖 |
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| 作者单位： | 1. 西安交通大学医学院人体解剖学教研室,陕西西安,710061
2. 西安华广生物工程公司,陕西西安,710005 |
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| 基金项目： | 国家自然科学基金资助 (No :30 30 0 395) |
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| 摘 要： | 目的 制备稳定分泌β -淀粉样肽 1～ 4 2 (Aβ1-42 )单克隆抗体的杂交瘤细胞 ,产生高效价抗体。方法 通过基因工程技术 ,将Aβ1-42 基因融合于乙肝核心抗原 (HBcAg)的主要免疫优势区 (MIR) ,制备Aβ和HBcAg的融合蛋白 ,免疫BALB/c小鼠 ,取脾细胞与SP2 / 0细胞融合 ,筛选能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞。结果 得到两株能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞 ,Aβ1-42 单克隆抗体的Ig亚类为IgG3 。结论 制备得到的Aβ1-42 单克隆抗体特异性强 ,效价高
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| 关 键 词： | β淀粉样肽 单克隆抗体 融合蛋白 细胞融合 |
| 文章编号： | 1671-8259(2004)05-0517-03 |
| 修稿时间： | 2004年2月24日 |
Preparation of monoclonal antibodies against β-amyloid peptide1-42 |
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| Dong Weijiang,Hu Haitao,Feng Gaifeng,Yang Guangxiao,Wang Quanying.Preparation of monoclonal antibodies against β-amyloid peptide1-42[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2004,25(5):517-519. |
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| Authors: | Dong Weijiang Hu Haitao Feng Gaifeng Yang Guangxiao Wang Quanying |
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| Abstract: | Objective To prepare a hybridoma secreting stab le monoclonal antibodies against β-amyloid peptide (Aβ 1-42) with high titer. Methods By genetic engineering technology, A β gene was recombined with the MIR of HBcAg to get the Aβ and HBcAg fusi on protein. Spleen cells from BALB/c mice immunized with Aβ and HBcAg f usion protein were fused with mouse myeloma cells SP2/0. Results Two strains of hybridomas (1H 7 and 1F 3) secreting stable monoclonal antibodies raised against Aβ 1-42 were ob tained. The subtypes of Aβ 1-42 antibodies were IgG 3. C onclusion The Aβ 1-42 monoclonal antibodies obtained have high titers and specificity. |
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| Keywords: | Aβ 1-42 monoclonal antibody (mAb) f usion protein cell fusion |
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