大鼠海马结构内生长抑素样神经元的衰老变化

结合免疫金银染色法及图像定量分析,观察了幼年(2个月)、中年(10个月)和老年(24个月)(各12只)wistar雄性大鼠海马结构内生长抑素(SOM)样神经元的衰老变化,结果发现,SOM样神经元的数量随增龄而减少,且各年龄组间存在高度显著差异(P<0.01)。中年组SOM样神经元胞体面积显著增大(P<0.01)。老年时的胞体有轻度萎缩现象,且边缘不规整,突起仲长增粗,但边缘粗糙,有断裂现象。SOM样神经元的灰度值随增龄而升高,提示神经元的SOM含量和活性在衰老进程中是降低的。

CHANGES IN SOMATOSTATIN-LIKE NEURONS OF THE HIPPOCAMPAL FORMATION IN AGING RATS

Combining the immunogold-silver staining (IGSS) method with the image quantitative analytical study, changes in somatostatin(SOM)-like, neurons of the hippocampal formation were investigated in young (2 monthes), adult (10 monthes) and old (24 monthes) rats. The results were as follows: 1. The number of the SOM-like neurons decreased gradually from the young to the old. Moreover, there was a significant difference among the three age groups (p<0.01). 2. The size of the SOM-like neuronal somas enlarged significantly (p<0.01) in the adult but had slight atrophy in the old. The SOM-like neurons in old had irregular edges with longer and fragmental processes. 3. The gray value of the SOM-like neurons rose gradually from the young to the old. It indicated that the somatostatin content and the activity of the neurons declined continually with aging.