CALCITONIN GENE-RELATED PEPTIDE INDUCES FIBROBLASTS FROM HIP PSEUDOCAPSULE TO PRODUCE CYTOKINES |
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| Authors: | QIAN Ye-bin ZENG Bing-fang ZHANG Xian-long JIANG Yao |
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| Institution: | QIAN Ye-bin(Department of Orthopaedic Surgery, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China)；ZENG Bing-fang(Department of Orthopaedic Surgery, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China)；ZHANG Xian-long(Department of Orthopaedic Surgery, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China)；JIANG Yao(Department of Orthopaedic Surgery, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China)； |
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| Abstract: | Objective To investigate calcitonin gene-related peptide (CGRP) involvement in the process of aseptic loosening. Methods We isolated fibroblasts from periprosthetic pseudocapsular tissue at the time of revision hip arthroplasty performed due to aseptic loosening. Fibroblasts were incubated for 24 h in the presence of CGRP, and the levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in the media were determined using enzyme-linked immunosorbent assay kit. Results We found that the levels of IL-1β, IL-6, and TNF-α increased in a time- and concentration-dependent manner. ConclusionThese inflammatory cytokines play an important role in the development of periprosthetic osteolysis and implant loosening. Our results suggest that CGRP may be involved in the pathogenesis of aseptic loosening. |
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