THE CYTOTOXIC EFFECTS OF CRUDE BILE ON HUMAN PANCREATIC CANCER CELL LINES |
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| Authors: | Lü Yi Wang Zifa Wang Bo Eiji Uchida Masahiko Onda Pan Cheng'en |
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| Affiliation: | 1. Department of Hepatobiliary Surgery, First Affiliated Hospital, Xi' an Medical University, Xi' an 710061,
2. Department of Hepatobiliary Surgery, First Affiliated Hospital, Xi' an Medical University, Xi' an 710061,China |
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| Abstract: | Objective To identify effects of bile acids on pancreatic cancer, The ultrastructure and growth of PANC-1 and MIA PaCa-2 cell lines in crude bile modified medium were studied. Methods The growth of PANC-1 and MIA PaCa-2 cells in RPMI 164o with or without 1%, 2% and 4% of the purified crude bile (containing total bile acids 1o. 17mmol/L) was assessed for 2, 4, 6, 8d by using MTT assay to determine inhibitory rate- The cell surface and intracellular ultrastructure of PANC-1 cells was investigated by SEM and TEM at 24h and 48h, respectively. Results The proliferation of both cell lines in bile treated medium were greatly retarded (P
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| Keywords: | bile acids extrahepatic cholestasis cytotoxicity pancreatic cancer cell line |
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