| THE CONSTRUCTION OF LEUKARYOTIC EXPRESSION PLASMID PCDNA3/HPV16E6 AND EXPERIMENTAL OBSERVATION OF NAKED DNA INJECTED INTO ANIMAL |
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| 作者姓名： | 许有元 王一理 司履生 孙向乐 |
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| 作者单位： | Department of Immunopathology，Xi'an Medical University，Xi'an 710061 |
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| 摘 要： | Cervicalcancerisacommonmalignancyofmarriedwomen.HighriskHPV(HPV16,18)infectioniscloselyassociatedwithcervicalcancer(l).HPV16DNAcanbedetectedinapproximately50%~90?rvicalcarcinomastZ).HPV16E6andE7geneshasbeenprovedtobetransforminggenes.TheirgeneproductscanbindtoandinactivateP53andPRbrespectively,playingacausalroleinthedevelopmentofcervicalcarcinomat27.AlotofinvestigationindicatethatHPV16E6/E7wereconsistentlyandhighlyexpressedincervicalcarcinomas,andthatE6/E7proteinscouldinducebothhu…
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THE CONSTRUCTION OF LEUKARYOTIC EXPRESSION PLASMID PCDNA3/HPV16E6 AND EXPERIMENTAL OBSERVATION OF NAKED DNA INJECTED INTO ANIMAL |
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| Xu Youyuan,Wang Yili,Si Lusheng,Sun Xiangle.THE CONSTRUCTION OF LEUKARYOTIC EXPRESSION PLASMID PCDNA3/HPV16E6 AND EXPERIMENTAL OBSERVATION OF NAKED DNA INJECTED INTO ANIMAL[J].Academic Journal of Xi’an Jiaotong University,1998(2). |
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| Authors: | Xu Youyuan Wang Yili Si Lusheng Sun Xiangle |
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| Abstract: | HPV16 infection is ciosely associated with cervicai carcinomas. A lot or investigation indicate that HPV16 E6/E7 proteins can act as tumor rejection antigens. So an HPV16E6 leukaryoticexpression plasmid pcDNA3/E6 was constructed and transfected Into Cos7 cells, which expressed E6protein in vitro. Animal experiments proved that intramuscular immunlzation with plasmid peDNA3/E6 could elicit specific immunoresponse against HPV16 E6 in mice. All these results suggest that E6DNA vaccine can serve as a candidate therapeutic vaccine for HPV16 associated diseases in humans. |
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| Keywords: | DNA vaccine IHPV16E6 cervical cancer |
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