| PCR法检测乙型肝炎患者外周血单个核细胞中HBV DNA |
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| 引用本文： | 陈太平,张树林,赵英仁,吴广利,严玉兰,李义方.PCR法检测乙型肝炎患者外周血单个核细胞中HBV DNA[J].西安交通大学学报(医学版),1994(2). |
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| 作者姓名： | 陈太平 张树林 赵英仁 吴广利 严玉兰 李义方 |
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| 作者单位： | 西安第一临床医学院肝炎研究室 |
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| 摘 要： | 采用ＰＣＲ法检测了７７例乙肝患者外周血单个核细胞（ＰＢＭＣ）中ＨＢＶＤＮＡ，２７例阳性，总阳性率为３５．１％。慢性活动性肝炎（９／２１，４２．９％），肝炎后肝硬化（９／１９，４７．４％）及重型肝炎（８／１６，５０．０％）患者ＰＢＭＣＨＢＶＤＮＡ阳性率明显高于慢迁肝（１／１４，７．１％）和急肝（０／７）患者。结果提示ＰＢＭＣ中ＨＢＶＤＮＡ的存在在乙肝发病机理中具有一定意义。
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| 关 键 词： | 多聚酶链反应 乙型肝炎病毒 乙型肝炎病毒DNA |
DETECTION OF HBVDNA IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF PATIENTS WITH HEPATITIS B BY POLYMERASE CHAIN REACTION |
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| Chen Taiping, Zhang Shulin, Zhao Yingren, et al.DETECTION OF HBVDNA IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF PATIENTS WITH HEPATITIS B BY POLYMERASE CHAIN REACTION[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1994(2). |
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| Authors: | Chen Taiping Zhang Shulin Zhao Yingren |
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| Abstract: | By means of polymerase chain reaction(PCR) technique, seventy- seven patients with various clinical types of hepatitis B were studied for the detection of hepatitis B virus (HBV) DNA in peripheral blood mononuclear cells (PBMCs).Twenty-seven cases were positive for PBMC- associated HBV DNA(35.1%).The positive rate of PBMCassociated HBV DNA in patients with chronic active hepatitis(9/21, 42.9%), posthepatitic cirrhosis(9/19,47.4%), and severe hepatitis(8/16,50.0%)was significantly higher than that in patients with chronic persistent hepatitis(1/14,7.1%)and acute hepatitis(0/7).These findings suggest that the existence of HBV DNA in PBMCs might play some roles in the pathogenesis of hepatitis B. |
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| Keywords: | polymerase chain reaction hepatitis B virus hepatitis B virus DNA |
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