P21~(waf1/cip1)和P53在子宫颈鳞状细胞癌中的表达及意义

目的　探讨P2 1waf1/cip1和P5 3基因与子宫颈癌发生发展的关系以及它们之间的相互关系。方法　采用免疫组化方法研究 15例慢性子宫颈炎和 47例子宫颈癌P2 1waf1/cip1和P5 3的表达情况。结果　P5 3蛋白在慢性子宫颈炎中阳性表达率为 0 ,子宫颈癌中阳性表达率为 38 3%,其P5 3蛋白异常表达与肿瘤发生、肿瘤细胞分化程度、临床分期有关。P2 1waf1/cip1在慢性子宫颈炎中阳性表达率为 86 7%,子宫颈癌中阳性表达率为 5 7 5 %,其阳性表达率降低与肿瘤发生、肿瘤细胞分化程度相关。结论　在子宫颈癌中P2 1waf1/cip1可通过P5 3依赖及非P5 3依赖两种途径表达。

Expression and significance of P53 and P21waf1/cip1 in cervical squamous cell carcinoma

Objective To determine the expression of P53 and P21 waf1/cip1 in cervical cancer and its relationship to development of the carcinoma. Methods Fifteen cases of chronic cervicitis and 47 cases of invasive cervical carcinoma were studied to determine expression of P53 and P21 by using an immunohistochemical method.Results The results indicated that P53 was detected in 18 specimens(38.3%) and overexpression of P53 was not only related to the development and progression of cervical cancer, but also related to the degree of differentiation of cancer cells and clinical stage. P21 waf1/cip1 was detected in 27 specimens(57.4%). The underexpression of P21 waf1/cip1 was not only associated with carcinogenesis of the cervix, but also associated with the degree of differentiation of cancer cells. There was an inverse relationship between P21 and P53 expression in tumors.Conclusion This study supports the hypothesis that P21 expression is regulated by P53 and that it is also regulated by an additional pathway in cervical carcinoma.