| 应用PCR方法检测人乳头瘤病毒的E_7转化基因 |
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| 引用本文： | 杜忆华,楚雍烈,司履生,李明众,刘汉馨.应用PCR方法检测人乳头瘤病毒的E_7转化基因[J].西安交通大学学报(医学版),1994(3). |
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| 作者姓名： | 杜忆华 楚雍烈 司履生 李明众 刘汉馨 |
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| 作者单位： | 西安基础医学院分子病毒研究室 |
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| 摘 要： | 应用聚合酶链反应技术（ＰＣＲ）检测５４份子宫颈癌活检标本中人乳头瘤病毒１６型（ＨＰＶ－１６）Ｅ７转化基因的存在情况，结果显示阳性率为７２．２％（３９／５４）。ＨＰＶ－１６Ｅ７转化基因在人子宫颈癌组织中的高检出率，证实该转化基因与其发生关系密切。本研究同时证明，ＰＣＲ方法具有敏感、特异、快速和实用特点，值得推广。
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| 关 键 词： | 人乳头瘤病毒，子宫颈癌，聚合酶链反应，转化基因 |
DETECTION OF E_7 TRANSFORMING GENE OF HUMAN PAPILLOMAVIRUS TYPE 16 IN BIOPSIES OF CERVICAL CANCER WITH POLYMERASE CHAIN REACTION |
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| Du Yibua,Chu Yonglie,Shi Lisheng,et al.DETECTION OF E_7 TRANSFORMING GENE OF HUMAN PAPILLOMAVIRUS TYPE 16 IN BIOPSIES OF CERVICAL CANCER WITH POLYMERASE CHAIN REACTION[J].Journal of Xi‘an Jiaotong University:Medical Sciences,1994(3). |
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| Authors: | Du Yibua Chu Yonglie Shi Lisheng |
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| Institution: | Dept.of Microbiology Xi'an 710061 |
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| Abstract: | The E7 open reading frame(ORF )of human papillomavirus type 16(HPV-16 )plays an important role in cell transformation. In order to study the relationship between HPV-16 E7 ORF and cervical cancer, the 54 biopsies from patients with cervical cancer were detected by using polymerase chain reaction(PCR).THe results showed that the positive rate which detected HPV-16 E7 ORF was much higher, the positive rate 72.2%(39/54 ) and HPV-16 E7 transforming gene was closely associated with cervical cancer. This study also indicated that PCR was more sensitive, specific and valuable. |
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| Keywords: | human papillomavirus cervical cnacer E_7 ORF PCR |
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