萘丁美酮对十二指肠胃返流所致胃黏膜损伤保护作用的实验研究

目的探讨非甾体类消炎药萘丁美酮对十二指肠返流导致返流性胃炎的治疗作用。方法用SD大鼠建立十二指肠胃返流模型,40只模型大鼠等分为萘丁美酮组和西沙比利组、铝碳酸镁组、空白手术组;另取10只SD大鼠作为假手术组。干预时间12周。对模型大鼠的胃标本进行病理学检查,采用免疫组化方法对胃黏膜的COX2和TNFα进行染色。结果萘丁美酮组的病理改变明显轻于空白手术组(P<0.01)而又重于假手术组(P<0.05),与铝碳酸镁组和西沙比利组相比则差异不显著(P>0.05)。TNFα的免疫组化染色显示,萘丁美酮组与其他各组间均无显著性差异(P>0.05)。COX2的免疫组化染色显示,萘丁美酮组与空白手术组相比COX2表达明显降低(P<0.01),与铝碳酸镁组、西沙比利组和假手术组相比则无显著性差异(P>0.05)。结论在十二指肠返流导致的返流性胃炎中,萘丁美酮有明显的治疗效果。萘丁美酮可明显降低胃黏膜组织中COX2水平。

The experimental study of the protective function of nabumetone on the injury of gastric mucosa caused by duodenogastric reflux

Objective To investigate the therapeutic effects of nabumetone on bile-reflux gastritis caused by duodenogastric reflux. Methods Sprague-Dawley rats were used to establish duodenogastric reflux model. A total of 40 survived model rats were averagely divided into four groups: nabumetone, hydrotalcite, cisapride and operation control groups. Another 10 SD rats were selected as sham operation control and the intervention period was 12 weeks. The pathological study of gastric specimen was performed; COX-2 and TNF-α in the mucosa membrane were immunohistochemically examined. Results The histological changes in nabumetone group was apparently less than that in operation group(P< 0.01 ), but distinctively severer than that in sham operation group(P< 0.05 ), and there was no significant difference between nabunetone group and hydrotalcite and cisapride group(P> 0.05 ). As for TNF-α, the score in nabumetone group was of no significant difference compared with other group(P> 0.05 ). On the contrary, the COX-2 score in nabumetone group was obviously lower than that in the operation group (P< 0.01 ), and no significant difference was found between nabumetone group and hydrotalcite, cisapride and sham operation groups(P> 0.05 ). Conclusion Nabumetone has obvious therapeutic effects on bile-reflux gastritis caused by duodenogastric reflux. Nabumetone can significantly decrease the level of COX-2 in the gastric mucus membrane.