妊娠滋养细胞疾病P16和P53蛋白表达及意义

目的　探讨P1 6、P5 3基因产物在正常绒毛组织、葡萄胎、恶性葡萄胎 (恶葡 )、绒毛膜上皮癌 (绒癌 )中的表达与恶性程度及预后的关系。方法　采用SP免疫组织化学技术对正常绒毛组织、葡萄胎、恶葡、绒癌组织中的肿瘤抑制基因P1 6、P5 3蛋白表达进行检测。结果　正常绒毛组织、葡萄胎、恶葡、绒癌组织中P1 6蛋白阳性率分别为 1 0 0 % (1 0 /1 0 )、75 % (3 0 /40 )、41 .7% (5 /1 2 )、2 2 .2 % (2 /9)呈递减趋势。正常绒毛组织与葡萄胎之间以及葡萄胎、恶葡、绒癌之间差异有显著性 (P <0 .0 5 ) ;正常绒毛组织P5 3蛋白不表达 ,葡萄胎表达呈弱阳性 3 0 % (1 2 /40 ) ,恶葡为83 .3 % (1 0 /2 0 ) ,绒癌为 88.9% (8/9) ,显著高于正常绒毛组织和葡萄胎 (P <0 .0 5 )。正常绒毛、葡萄胎、恶葡和绒癌中P1 6和P5 3蛋白表达之间未见明显相关。结论　恶葡及绒癌组织中P1 6蛋白低表达和P5 3蛋白过表达均有促进肿瘤细胞增殖作用 ,与滋养叶细胞肿瘤恶性程度及预后有关

Expression of P16 and P53 proteins in gestational trophoblastic diseases

Objective To investigate the correlation of P16 and P53 expressions between normal trophoblast, hydatidiform mole, invasive mole and choriocarcinoma. Methods With streptavidin peroxidase immunohistobiochemistry, P16 and P53 expressions were examined in normal trophoblast, hydatidiform mole, invasive mole and choriocarcinoma.Results Ten cases of normal trophoblast were P16 positive. Among the 40 cases of hydatidiform mole, 10 cases were P16 negative and 30 cases positive (75%). Among the 12 cases of invasive mole, 7 cases were P16 negative and 5 cases positive (41.7%). Among the 9 cases of choriocarcinoma, 7 cases were P16 negative and 2 cases were positive (22.2%). There was remarkable difference at the levels of P53 expression between invasive mole (30%), choriocarcinoma (88.9%) and normal trophoblast, and hydatidiform mole (30%). No significant correlation between the P16 and P53 expressions in normal trophoblast, hydatidiform mole, invasive mole and choriocacinoma.Conclusion Both P16 protein low expression and P53 protein overexpression play an important role in cell proliferation, and are related to malignancy and prognosis of trophoblastic tumors.