死亡相关蛋白激酶在乳腺癌进展过程中的表达及意义

目的检测乳腺癌组织中死亡相关蛋白激酶(DAPK)的表达,探讨DAPK在乳腺癌发生、发展中的作用。方法采用免疫组化SP法检测42例乳腺增生、30例乳腺不典型增生、14例乳腺非浸润性癌及68例乳腺浸润性癌的DAPK表达水平。结果乳腺浸润性癌组织中DAPK的阳性表达率为42.6%,明显低于乳腺增生(92.9%)、乳腺不典型增生(73.3%)、乳腺非浸润性癌组织中的表达(71.4%,均为P<0.05);非浸润性癌和不典型增生组织中的DAPK阳性表达率为72.7%,亦低于乳腺增生组织(P<0.05),但是非浸润性癌和不典型增生两组间无显著性差异(P>0.05)。研究还发现DAPK阳性表达与乳腺癌的雌激素受体的表达、淋巴结转移、病理分级、组织分型等相关。结论DAPK表达降低对乳腺癌的演化和进展具有一定重要作用,可用于乳癌的早期诊断及靶向治疗。

The expression of death-associated protein kinase in the progression of breast carcinoma and its significance

Objective To investigate the possible relationship between the expression of death-associated protein kinase(DAPK) and the breast tumor progression from premalignant in situ to invasive carcinoma.(Methods)The expression of DAPK was evaluated by using S-P immunohistochemistry method in 42 cases of breast hyperplasia,30 cases of breast atypical hyperplasia,14 cases of non-invasive breast carcinoma,and 68 cases of invasive breast carcinoma.The relationship between DAPK and the pathologically biological features of breast cancer was assessed.Results The positive rate of DAPK in invasive breast carcinoma was 42.6%,obviously lower than that in hyperplasia(92.9%),atypical hyperplasia(73.3%) or non-invasive carcinoma(71.4%) with statistical significance(P<0.05).The positive expression rate of DAPK in breast atypical hyperplasia and non-invasive carcinoma was 72.7%,which was lower than that in breast hyperplasia(P<0.05).But there was no significant difference between atypical hyperplasia and non-invasive carcinoma(P>0.05).There might be an association of the expression of DAPK with estrogen receptor,lymph node metastasis,histology and histological grading of breast carcinoma.Conclusion The results indicate that the reduced expression of DAPK may play an important role in the development and progression of breast carcinoma.The reduction of DAPK illustrates its potential clinical application as a tumor marker for diagnosis and therapy.