壶腹周围癌细胞核DNA含量测定与临床意义

在对 6 7例壶腹周围癌患者回顾性调查的基础上 ,采用 Feulgen′s染色方法和自动图像技术 ,定量测定了癌细胞核 DNA含量。结果 :(1) DNA非整倍体在壶腹周围癌中的发生率为 71.6 4 % ,且其发生率随组织学分级而上升 ;非整倍体肿瘤的预后远不如整倍体肿瘤 (P <0 .0 5) ;(2 )生存期≥ 3年和 <3年组的 DNA指数均值 (DI)有显著性差异 (P <0 .0 1) ;(3)按患者 DNA主干系峰位的 DNA指数值分为三种峰型 ,I型峰预后最好 , 型峰最差。提示 :DNA含量变化与组织学分级有密切关系 ,可作为壶腹周围癌常规临床病理的补充 ;非整倍体和 DNA指数 >3预示壶腹周围癌预后不良 ;DNA含量测定有助于判断预后 ,筛选高危复发转移病人 ,指导临床治疗。

Cell nuclear DNA content in periampullary carcinoma

patients with periampullary carcinoma were recruited.Based on retrospective study.Feulgen′s staining and automatic imaging analysis technique were adopted to evaluate cell nuclear DNA content.The results showed that :①The incidence of aneuploid in periamupullary carcinoma was 71.64%, which increased with advanced histologic stage.Aneuploid tumor had a poorer prognosis when compared with diploid tumor (P<0.05);②The means of DNA index was higher in overall survival period ≥3 years group than those <3 yesrs group (P=0.001);③Based on the DNA index values of main peak position of DNA stem bars, the distribution pattern of DNA population in PAC could be divided into three types. Among them, type I had the best prognosis, whiletype Ⅲ had the worst.So we say in periampullary carcinoma,①DNA content is associated with the histologic stage, therefore it can be used as a supplement of the usual clinical pathology;②Anueploid and DNA index≥3 may predict a poor prognoiss;③analysis estimation of DNA is helpful in predicting the prognosis, selecting the high risk recurrent and metastatic patients and directing comprehensive therapy.