肺吸入胰岛素气雾剂对实验性糖尿病大鼠的治疗作用

目的 观察肺吸入胰岛素气雾剂对糖尿病大鼠血糖的影响。方法 用四氧嘧啶静脉注射诱导糖尿病大鼠模型，以甘氨胆酸钠及甘珀酸钠为吸收促进剂，分别制成肺吸入胰岛素气雾剂，糖尿病大鼠气管插管后用药，测定血糖。结果 糖尿病大鼠模型为低胰岛素分泌型。甘氨胆酸钠作吸收促进剂的气雾剂组在给药后15min起效，30min血糖下降百分数达峰值；甘珀酸钠为吸收促进剂的气雾剂在给药后15min起效，2h血糖下降百分数达峰值；对照的皮下注射胰岛素组在用药后1h血糖下降百分数达峰值。气雾剂用量增加，糖尿病大鼠血糖下降百分数无明显增加。结论 肺吸入胰岛素气雾剂有明显的降血糖作用。

Therapeutic effect of pulmonary inhaled insulin aerosol on rats of experimental diabe tes

Objective\ To observe the effect of reducing glycemia o n rats suffering diabetes mellitus(DM).Methods\ We induced rats suff ering DM by subcutaneous injecting tetraoxypyrimidine.Natrium glycocholate and n atrium glycerate were chosen as respiratory tract musoca inhalant enhancer,and w e re made into inhaled insulin aerosol each other.The aerosol including natrium gl ycocholate and natrium glycerate were administered to rats suffering DM after ca theterization of bronchus.The level of glycemia was tested.Results\ We confirmed that rats suffering DM was a lowsecretary insulin DM model.The ae r osol that natrium glycocholate as inhalant enhancer acted in 15min and peaked in 30min after administration.The aerosol that natrium glycerate as inhalant enhan cer acted in 15min and peaked in 2h after administration.The group subjected to subcutaneous injection insulin peaked in 1h after administration.The aerosol men tioned above were administrated to rats with DM in different dose of 5IU/kg,10IU /kg,15IU/kg.We observed that there was no significant enhancement of glycemia de creasing percent.Conclusion\ The pulmonary inhaled insulin aerosol w hich we made can obviously reduce glycemia.