P53与P21(WAF1/CIP1)在甲状腺癌中的表达及临床意义

目的　探讨 P53、P2 1 (WAF1 /CIP1 )基因与甲状腺癌的发生、发展及预后的关系以及它们的相互关系。方法　应用 S- P免疫组化技术 ,检测 60例甲状腺癌、1 0例正常甲状腺组织、1 0例结节性甲状腺肿、1 0例甲状腺腺瘤组织中 P53、P2 1 (WAF1 /CIP1 )的表达。结果　 P53、P2 1(WAF1 /CIP1 )阳性反应仅见于甲状腺癌 ,其阳性率分别为 53.3%、41 .7% ;P53阳性率在未分化癌、有淋巴结转移或 、 期病例中增高 (P <0 .0 5) ;但 P2 1 (WAF1 /CIP1 )阳性率在未分化癌、有淋巴结转移或 、 期病例中降低 (P <0 .0 5) ;P53表达与 P2 1 (WAF1 /CIP1 )表达关系密切(P <0 .0 5)。结论　 P53突变、P2 1 (WAF1 /CIP1 )缺乏均与甲状腺癌发生有关 ;P2 1 (WAF1 /CIP1 )在甲状腺癌中表达主要依赖于 P53;P53、P2 1 (WAF1 /CIP1 )在甲状腺癌中表达的变化可用于评估其生物学行为和判断预后

Expression and significance P53 and P21(WAF1/CIP1) in thyroid carcinoma

Objective To determine expression of P53 and P21(WAF1/CIP1) in thyroid carcinomas and its relationship to development and prognosis of the carcinoma.Methods 90 cases of thyroid tissues (60 thyroid carcinomas,10 thyroid adenomas, 10 goitres and 10 normal thyroid tissues) were studied by SP immunohistochemical method.Results Positive immunoreactivity of P53 and P21(WAF1/CIP1) was found only in thyroid carcinomas, the positive rate of the P53 and P21 is 53.3% and 41.7%.The positive staining rates of P53 were higher in cases of undifferentiated carcinomas, positive metastasis lymph nodes or at tumor stage Ⅲ,Ⅳ than in the cases of well differetiated, no metastasis lymph nodes, or at tumor stage I,Ⅱ. In addition to, the positive staining of P21(WAF1/CIP1) were lower in cases of undifferentiated carcinomas, positive metastasis lymph nodes or at tumor stage Ⅲ,Ⅳ than in the cases of well differetiated, no metastasis lymph nodes, or at tumor stage Ⅰ,Ⅱ .The P21(WAF1/CIP1) expression rate in the P53 positive group was lower than that of the P53 negative group (P<0 05).Conclusion The expression of P21(WAF1/CIP1) protein in thyroid cancer is related with P53 depend pathway and P53 independent pathway, mainly the P53 depend pathway.Examination of expression of P53 and P21(WAF1/CIP1) proteins may be helpful to judge the thyroid cancer′s behavior and prognosis.