龈沟液中MMP-2,9的量与牙周炎的关系

目的观察慢性牙周炎患者在牙周基础治疗前后龈沟液(GCF)中基质金属蛋白酶2,9(MMP2,9)水平的变化。方法采用滤纸条的袋内取样法取40例患者40个牙位治疗前(BT组)、治疗后(AT组)的GCF样本,同时取40例健康人的40个牙位的GCF样本,用酶联免疫法(ELISA)检测其中的MMP2,9的水平。结果牙周基础治疗后GCF中MMP2,9的总量均较治疗前显著下降(P<0.01),治疗前后MMP2,9的总量均与对照组有显著差别(P<0.01),治疗前后GCF中MMP2与MMP9的含量呈显著正相关(P<0.01)。结论GCF中MMP2,9的总量在牙周基础治疗后降低,其水平反映牙周组织的破坏和炎症程度,可作为评价疗效的客观指标。

Correlation of matrix metalloproteinase-2, 9 in gingival crevicular fluid with periodontal inflammation

Objective To examine the changes of matrix metalloproteinase-2,9 (MMP-2,9) in gingival crevicular fluid(GCF) after phase 1 periodontal treatment of adult patients with periodontitis. Methods GCF was sampled with filter paper strips by intra-pocket method to determine MMP-2,9 levels. Forty teeth of forty adult patients with periodontitis and forty teeth of forty periodontally healthy persons were included in this study. Assays for MMP-2,9 in GCF were performd by ELISA. Results Contents of MMP-2,9 were higher in AT group than those in controls(P<0.01); levels of MMP-2,9 were decreased in AT compared with those in BT(P<0.01). The content of MMP-2, 9 in GCF had significant correlations. Conclusion Levels of MMP-2, 9 in GCF decreased after phase 1 periodontal treatment. MMP-2, 9 could act as objective parameters to evaluate the effect of periodontal therapy.