不同剂量氟中毒大鼠剩余牙槽骨骨改建的研究

目的观察氟中毒对大鼠剩余牙槽骨的影响。方法选用45d龄SPF大鼠60只,随机分为4组,对照组(饮用蒸馏水)、低剂量氟中毒组(饮水F-浓度为50mg/L)、中剂量氟中毒组(饮水F-浓度为100mg/L)和高剂量氟中毒组(饮水F-浓度为200mg/L),饲养2月后拔除全部左侧上颌磨牙;饲养至拔牙1月后和2月后时,取左侧上颌剩余牙槽骨;采用酶组织化学方法观察不同浓度氟中毒大鼠剩余牙槽骨中碱性磷酸酶的表达情况。结果对照组和低剂量氟中毒组碱性磷酸酶(ALP)表达较显著,成骨细胞较多;而中、高剂量氟中毒组成骨细胞数量较少,表达较弱;与拔牙1月后相比,拔牙2月后各组剩余牙槽骨中ALP表达减少。表明剩余牙槽骨骨组织随着时间的推移,成骨细胞数量减少,骨形成减少。结论长期氟中毒对大鼠剩余牙槽骨存在实质性损伤。

Research on residual alveolar bone in fluorosis rats

Objective To evaluate the effect of fluorosis on residual alveolar bone of rats.Methods Sixty SPF rats aged 45 days were randomly divided into four groups: control group,low-dose group,medium-dose group and high-dose group.Rats in the four groups took normal food and drank distilled water,sodium fluoride of 50 mg/L,100 mg/L and 200 mg/L,respectively.All molars in left maxillary bone were extracted after 2 months.Each rat in the four groups was killed at one or two month after tooth extraction.The mandible residual bone of each rat was removed to evaluate the expression of alkaline phosphatase(ALP) with histochemical staining.Results The results demonstrated that the positive expression of ALP was significantly elevated with the increase of osteoblasts in control group and low-dose group,but decreased with the decline of osteoblasts in medium-dose group and high-dose group.The decline of the counted number of positive cells showed that the expression significantly lowered in high-dose group.Compared with that at one month,the counted number of positive cells at month two after tooth extraction was significantly lower in all drug groups.Conclusion Chronic fluoride poisoning accelerates the damage of alveolar bone in rats.