蟾蜍灵对顺铂耐药胃癌SGC7901细胞增殖与凋亡的影响及其作用机制

目的探讨蟾蜍灵(bufalin)对顺铂耐药胃癌细胞(SGC7901/CDDP)增殖及凋亡的影响及其作用机制。方法采用MTT比色法检测bufalin对SGC7901/CDDP细胞增殖的抑制作用;应用流式细胞术PI染色检测细胞凋亡;应用Western blot检测凋亡相关蛋白Bcl-2、Bax的表达。结果 Bufalin以时间和剂量依赖方式抑制SGC7901/CDDP细胞增殖,24、48、72h的IC50值分别为106.3、53.8、10.9nmol/L。选取10、50、100nmol/L的bufalin作用SGC7901/CD-DP细胞24h,凋亡率分别为7.3%、12.6%和26.4%,呈浓度依赖性。Western blot分析结果表明,SGC7901/CDDP细胞经10、50、100nmol/L的bufalin处理后,促凋亡基因Bax表达逐渐上调,抗凋亡基因Bcl-2表达逐渐减弱。结论 Bufalin在体外能够抑制对顺铂耐药的胃癌细胞(SGC7901/CDDP)的增殖,且通过调控Bcl-2、Bax的表达而诱导细胞凋亡。

Effects of bufalin on proliferation and apoptosis of SGC7901/CDDP and its underlying mechanisms

Objective To investigate the effects of bufalin on proliferation and apoptosis of cisplatin-resistant gastric cancer cell(SGC7901/CDDP) in vitro and the underlying mechanisms.Methods Cell proliferation was measured using MTT assay and cell apoptosis was determined by flow cytometry after propidium iodide staining.The expressions of Bcl-2 and Bax proteins were detected by Western blot.Results Bufalin could inhibit the proliferation of SGC7901/CDDP in the time-and dose-dependent manners.The concentration of inhibited cell viability(IC50) for 24,48 and 72 h was 106.3,53.8 and 10.9 nmol/L,respectively.After treatment with bufalin of 10,50 and 100 nmol/L for 24 h,the rate of SGC7901/CDDP apoptosis was 7.3%,12.6% and 26.4%,respectively,which was concentration-dependent.When the cell was treated with bufalin of different concentrations,the expression of Bax was up-regulated but the expression of Bcl-2 was down-regulated.Conclusion Bufalin can inhibit the proliferation of cisplatin-resistant SGC7901/CDDP.The cell apoptosis is related to the up-regulated expression of Bax and the down-regulated expression of Bcl-2.