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| Ⅰ型胶原蛋白基因在反流性食管炎、Barrett食管及食管腺癌中的表达 | |
| 引用本文： | 赵俊杰,雷艳霞,康丽丽,赵晶,李亚清,刘晓庆,任春蓉.Ⅰ型胶原蛋白基因在反流性食管炎、Barrett食管及食管腺癌中的表达[J].西安交通大学学报(医学版),2012,33(4):443-445,477. |
| 作者姓名： | 赵俊杰 雷艳霞 康丽丽 赵晶 李亚清 刘晓庆 任春蓉 |
| 作者单位： | 1. 西安交通大学医学院环境与疾病相关基因教育部重点实验室,陕西西安,710061 2. 长安区医院,陕西西安,710100 3. 西安交通大学医学院第二附属医院,陕西西安,710004 |
| 基金项目： | 陕西省科技攻关项目(No.2006k10G4)~~ |
| 摘 要： | 目的探讨反流性食管炎、Barrett食管及食管腺癌黏膜组织中Ⅰ型胶原蛋白(COL1A1)基因mRNA的表达水平及意义。方法经胃镜取材,采用荧光定量PCR方法检测反流性食管炎、Barrett食管、食管腺癌组织中COL1A1基因mRNA的表达水平。结果 COL1A1mRNA在正常组和反流性食管炎阴性组食管黏膜中呈低表达(0.154±0.119,0.152±0.105),在Barrett食管与食管腺癌食管黏膜中呈明显的高表达(0.396±0.170,0.726±0.561)。并且在从正常→反流性食管炎→Barrett食管→食管腺癌的发生、发展过程中,COL1A1mRNA的表达呈现渐进性增强,并与病期有关。结论 COL1A1基因的异常表达与反流性食管炎、Barrett食管和食管腺癌的发生、发展密切相关;COL1A1mRNA的表达水平可用作监测Barrett食管和食管腺癌早期发生及干预治疗效果的有用指标。 |
| 关 键 词： | 反流性食管炎 Barrett食管 食管腺癌 Ⅰ型胶原蛋白基因 |
Expression of Col1A1 protein in reflux esophagitis,Barrett esophagus and esophageal adenocarcinoma |
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| ZHAO Jun-jie , LEI Yan-xia , KANG Li-li , ZHAO Jing , LI Ya-qing , LIU Xiao-qing , REN Chun-rong.Expression of Col1A1 protein in reflux esophagitis,Barrett esophagus and esophageal adenocarcinoma[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2012,33(4):443-445,477. | |
| Authors: | ZHAO Jun-jie LEI Yan-xia KANG Li-li ZHAO Jing LI Ya-qing LIU Xiao-qing REN Chun-rong |
| Institution: | 1.Key Laboratory of Environment and Genes Related to Diseases of Ministry Education,Medical School of Xi’an Jiaotong University,Xi’an 710061; 2.Chang’an District Hospital,Xi’an 710100;3.Department of Gastroenterology, the Second Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710004,China) |
| Abstract: | Objective To investigate the expression of Col1A1 mRNA in reflux esophagitis,Barrett esophagus and esophageal adenocarcinoma.Methods The expression level of Col1A1 mRNA in mucosa tissues of reflux esophagitis and Barrett esophagus and esophageal adenocarcinoma patients was detected by real-time fluorescent quantitative RT-PCR using SYBR Green I.Results The expression of Col1A1 mRNA was significantly higher in the Barrett esophagus(0.396±0.170) and esophageal adenocarcinoma tissues(0.726±0.561) than in the normal and reflux esophagitis esophageal tissues(0.154±0.119,0.152±0.105)(P<0.05;P<0.01).It also increased progressively with the development of reflux esophagitis →Barrett esophagus→ esophageal adenocarcinoma,and was related to staging.Conclusion The abnormal expression of Col1A1 mRNA is closely related to the pathogenesis and development of Barrett esophagus and esophageal adenocarcinoma.The expression of Col1A1 mRNA may be used as a valuable index for monitoring the early onset of Barrett esophagus and esophageal adenocarcinoma and intervening in therapeutic measure. |
| Keywords: | reflux esophagitis Barrett esophagus esophageal adenocarcinoma Col1A1 gene |
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