人体真菌还原硝酸盐作用的研究及其意义

为了研究人体所带真菌是否具有还原硝酸盐为亚硝酸盐的作用,从而研讨人类肿瘤病因中真菌因素的可能作用机理,我们用由妇女阴道及宫颈部分离出的多种真菌,进行了还原硝酸盐实验研究,并对其结果在医学上的意义作了讨论。实验结果表明短密青霉还原硝酸盐阳性率很高,略阳为72.73%(56/77),五峰为67.65%(23/34)。酵母及假丝酵母属真菌实验菌株数很少,还原阳性率也低。有些实验真菌如白曲霉、土曲霉、黄柄曲霉、屡地青霉、产紫青霉以及砖红酵母虽然还原阳性率很高,但由于这些真菌在综考中检出率很低,实验菌株数也少,故在肿瘤病因上的意义可能不大。

A TEST OF TRANSFORMING NITRATE WITH HUMAN FUNGI AND SIGNIFICANCE IN MEDICINE

We performed the transforming nitrate test mainly with penicillium brevi-compactum and other fungi. The strain of P. brevi-compactum and all the other fungi used for this test were isolated from patients with cervix uteri carcinoma, patients with gynopathy but no cancer, and the normal woman controls in Luoyang county of Shaanxi and in Wufeng county of Hubei respectively, which are the highest prevalance areas of cervix uteri carcinoma in China. The detection rate of P. brevicompactum is higher 72.73% (65/77) in Luoyang; 67.65 (23/34) in Wufeng. The detection rate of yeast and Candida is low. Though the detection rate of certain tested fungi such as A. serreus, A. candidus, P. cyelopium and Rhodotorula rubra is higher, these fungi are seldom found in the isolates from cervix uteri. Thus, these fungi might be no important significance in etiology of cervix uteri carcinoma. Because nitriamine is an important carcinogen and the effect of transforming nitrate to nitrite plays a more important role in the synthesis of nitriamine, P. brevi-compactum is the dominant strain of fungi isolated from the patient with cervix uteri carcinoma in this investigation. The higher detection rate of the treated P. brevi-compactum shows that this fungus plays a role in the etiology or incidence of cervis uteri carcinoma. To solve this difficult and complex problem of the role that fungal agent okay in the etiology of carcinoma further studies must be done.