反义C-myc寡核苷酸对人肝癌细胞SMMC-7721的生长抑制

针对C－mycmRNA第二外显子起站码AUG及下游4个密码子设计合成了反斗、正义及错配寡核苷酸（ODNS），并对合成的ODNs进行了疏代磷酸化修饰。在人肝癌细胞SMMC－7721培养体系中，对反义寡核苷酸（ASODN）抑制细胞增殖的作用进行了研究。发现①与正义和错配ODNs相比较，反义C－mycODN有明显抑制人肝癌细胞SMMC-7721生长的作用;②该生长抑制作用随ASODN剂量增加而增强，随ASODN作用时间而变化;③流式细胞计数仅测定证明反义C－mycODN主要阻断肝癌细胞增殖周期的G1到S期。结果提示：反斗C－mycODN在体外有序列特异性和细胞周期特异性抑制人肝癌细胞的生长作用，且该抑制作用与ASODN作用时间及剂量有关。

THE EFFECT OF ANTISENCE C-myc OLIGODEOXYNUCLEOTIDES ON GROWTH OF HUMAN SMMC-7721 HEPATOMA CELLS

We synthesized antisense, sense andmismatch Oh gode oxynuc leot ide s (ODNs ) accordingto starting code AUG and four codes downtream ofthe second exon of C-myc mRNA,and modified thesynthesised ODNs by thiophosphorylation. The inhibition of antisense ODNs (ASODN) of C-myc on thegrowth of human SMMC-7721 hepatoma cells wasobserved in culture. The results showed that: ① The ASODN had a significant inhibition of the growth ofhuman SMMC-7721 hepatoma cells,compared withsense and mismatch ODNS;② The effect of the inhibition increased with the increasing of the dosageof ASODN and changed with the time of ASODNI③ The measurements by Flow cytometry provedthat ASODN mainly blocked the G2 to S of the cycling of bepatoma cell. It was demonstrated that ASODN inhibited growth of human hepatoma cellswith sequence specificity and cycling specificity,andthe effect of the inhibition related to dosage and timeof ASODN.