| CONSTRUCTION OF HUMAN INTERLUEKIN-18 DNA VACCINE AND IT‘S EXPRESSION IN MAMMALIAN CELLS |
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| 引用本文： | 安小惠
,王一理
,来宝长
,耿一萍
,司履生.CONSTRUCTION OF HUMAN INTERLUEKIN-18 DNA VACCINE AND IT‘S EXPRESSION IN MAMMALIAN CELLS[J].西安交通大学学报(英文版),2001,13(2):156-158. |
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| 作者姓名： | 安小惠 王一理 来宝长 耿一萍 司履生 |
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| 作者单位： | An Xiaohui，Wang Yili，Lai Baochang，Geng Yiping，Si Lüsheng Department of Immunopathology，Medical School of Xi'an Jiaotong University，Xi'an 710061，China. |
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| 摘 要： | Ｈｕｍａｎｉｎｔｅｒｌｅｕｋｉｎ 18(ｈＩＬ 18)ｉｓａｎｅｗｌｙｉｄｅｎ ｔｉｆｉｅｄｃｙｔｏｋｉｎｅｍａｉｎｌｙ ｐｒｏｄｕｃｅｄｂｙｍｏｎｏｃｙｔｅｓａｎｄｍａｃｒｏｐｈａｇｅｓ.ＴｈｅｃｙｔｏｋｉｎｅｃａｎｉｎｄｕｃｅＴｈ1ｃｅｌｌｓｔｏｓｅｃｒｅｔｅＩＦＮ γ ,ＩＬ 2ａｎｄＧＭ ＣＳＦ .Ｉｔｅｎｈａｎｃｅｓｔｈｅｃｙ ｔｏｔｏｘｉｔｙｏｆＮＫａｎｄＣＴＬｃｅｌｌ,ａｎｄａｌｓｏｒｅｄｕｃｅｓｔｈｅｌｅｖ ｅｌｏｆＩＬ 10 .Ｔｈｕｓ,ｔｈｅｒｅｉｓｐｏｔｅｎｔｉａｌｔｈｅｒａｐｅｕｔｉｃｖａｌｕｅｏｆ…
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CONSTRUCTION OF HUMAN INTERLUEKIN-18 DNA VACCINE AND IT'S EXPRESSION IN MAMMALIAN CELLS |
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| An Xiaohui,WANG Yili,Lai Baochang,GENG Yiping,Si Lüsheng.CONSTRUCTION OF HUMAN INTERLUEKIN-18 DNA VACCINE AND IT'S EXPRESSION IN MAMMALIAN CELLS[J].Academic Journal of Xi’an Jiaotong University,2001,13(2):156-158. |
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| Authors: | An Xiaohui WANG Yili Lai Baochang GENG Yiping Si Lüsheng |
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| Abstract: | Objective To construct the human interleukin 18 DNA plasmids vaccine and to express the eukaryotic plasmids vaccine in mammalian cell lines Cos 7 and D5.Methods Gene recombinant technique was used to construct hIL 18 eukaryotic expression vectors.Calcium phosphate method was performed to transect recombinant hIL 18 eukaryotic expression vectors into Cos 7 and D5 cells. In situ hybridization and Western Blot were implemented to verify the transient expression of recombinant hIL 18 in Cos 7 and D5.Results The eukaryotic expression plasmid pVAX1 IL 18 was constructed successfully.hIL 18 was transiently expressed in Cos 7 and D5.Conclusion The eukaryotic expression plasmid pVAX1 IL 18 was constructed. In situ hybridization and Western Blot results proved the successful transient expression of pVAX1 IL 18 in Cos 7 and D5.Therefore,the work has settled the foundation for further biological research on hIL 18,including immunogene therapy through hIL 18. |
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| Keywords: | human interleukin 18 gene clone eukaryotic expression DNA vaccine |
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