| siRNA AGAINST SURVIVIN SUPPRESSES THE PROLIFERATION OF PANCREATIC CANCER CELL PC-2 AND INDUCES ITS APOPTOSIS |
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| 作者姓名： | 管海涛 薛兴欢 王西京 李昂 秦兆寅 |
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| 作者单位： | [1]Department of ＇ Oncosurgery , the Second Affiliated Hospital of Medical School, Xi＇an J iaotongUniversity, Xi＇an 710004, China. [2]Medical Research Center, Affiliated Stomatological Hospital of Medical School, Xi＇an Jiaotong University, Xi＇an 710004, China [3]Department of General Surgery, the Second Affiliated Hospital of Medical School, Xi＇an Jiaotong University, Xi＇an 710004, China |
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| 基金项目： | Foundation Item： This study was supported by the Foundation of Tackling Key Problems in Science and Technology of Shaanxi Province [No. 2004K13- G11 （ 1 ） ]. |
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| 摘 要： | Survivinis a member of the inhibitor of apop-tosis family,whichinhibits cell apoptosis and regu-lates cell division.Survivin is expressed in embry-onic tissues as well as in the majority of humancancers,but is not expressed in most nor mal adulttissues.The highly cancer-specific expression ofSurvivin,coupled withits i mportance in inhibitingcell apoptosis and in regulating cell division,makesit a useful diagnostic marker and ideal target forcancer treat ment1].RNAi is a process of post trans…
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| 关 键 词： | 胰腺肿瘤 肿瘤细胞 细胞凋亡 基因干扰 |
| 文章编号： | 1671-8267（2006）01-0078-05 |
siRNA AGAINST SURVIVIN SUPPRESSES THE PROLIFERATION OF PANCREATIC CANCER CELL PC-2 AND INDUCES ITS APOPTOSIS |
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| Guan Haitao,Xue Xinghuan,Wang Xijing,Li Ang,Qin Zhaoyin.siRNA AGAINST SURVIVIN SUPPRESSES THE PROLIFERATION OF PANCREATIC CANCER CELL PC-2 AND INDUCES ITS APOPTOSIS[J].Academic Journal of Xi’an Jiaotong University,2006,18(1):78-82. |
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| Authors: | Guan Haitao Xue Xinghuan Wang Xijing Li Ang Qin Zhaoyin |
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| Abstract: | Objective Blocking the expression of Survivin with siRNA, and the effects of suppressing the proliferation of PC-2 cell and inducing its apoptosls were investigated. Methods Constructed the siRNA a gainst Survivin plasmid expression vector and transfected it into PC-2 cell with lipofectamineTM 2000, the changes of Survivin expression were detected by semi-quanUUve RT-PCR and method, The effect of suppressing the proliferation of PC-2 cell was detected by the method of MTT; the effect of inducing PC-2 cell apoptesis was detected by flow cytometry. Results The sequence specific siRNA can effectively block the expression of Survivin both at the mRNA and protein levels, the expression inhibition ratio was 81.25% at mRNA level and 74.24% at protein level; blocking the expression of Survivin can suppress the proliferation of PC-2 cell significantly, 24, 48 hours after the cell was reseeded, the proliferation inhibition ratio was 28. 00% and 33. 38% respectively; 24, 48 hours after the transfection, 8.46 % and 7.53 % cells were induced to apoptosis respectively. Conclusion The siRNA against Survivin plasmid expression vector constructed in the study can blocking the expression of Survivin in PC-2 cell effectively and specifically; blocking the expression of Survivin can significantly suppress the proliferation of PC-2 cell and induce a certain degree cells apoptosls; RNAi against Survivin is of a certain value in the gene therapy of pancreatic cancer. |
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| Keywords: | RNA interfegna survivin pancreatic cancer |
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