| 氟化物对体外器官培养人牙胚细胞增殖及相关调控蛋白表达的影响 |
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| 引用本文： | 侯铁舟,李志丹,王强,李相如,王红,陶洪.氟化物对体外器官培养人牙胚细胞增殖及相关调控蛋白表达的影响[J].西安交通大学学报(医学版),2003,24(4):302-305. |
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| 作者姓名： | 侯铁舟 李志丹 王强 李相如 王红 陶洪 |
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| 作者单位： | 西安交通大学口腔医学院口腔内科,陕西西安,710004 |
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| 基金项目： | 陕西省科技攻关基金资助项目 (No .2 0 0 1K1 0 G9)，西安交通大学重点科研培植项目 (No.PZ0 98) |
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| 摘 要： | 目的 观察氟对牙胚细胞增殖及相关调控蛋白表达的影响。方法 采用人牙胚体外器官培养模型 ,组织学观察低浓度 (10mg·L- 1 )和高浓度 (2 5mg·L- 1 )氟对细胞增殖的影响 ;免疫组织化学及图像分析技术检测氟对细胞周期蛋白D1 (cyclinD1 )和细胞增殖核抗原 (PCNA)表达的影响。结果 氟处理组细胞增殖受抑 ,cyclinD1 和PCNA的表达显著降低 (P <0 .0 1)。结论 氟化物可能通过抑制cyclinD1 和PCNA的表达引起细胞增殖抑制 ,导致牙胚发育异常
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| 关 键 词： | 氟化物 牙胚 器官培养 细胞周期蛋白D1 细胞增殖核抗原 |
| 文章编号： | 1671-8259(2003)04-0302-04 |
| 修稿时间： | 2002年11月20 |
Effect of fluoride on human tooth-germ cell proliferation and expression of related regulators in organ culture in vitro |
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| Hou Tiezhou,Li Zhidan,Wang Qiang,Li Xiangru,Wang Hong,Tao Hong.Effect of fluoride on human tooth-germ cell proliferation and expression of related regulators in organ culture in vitro[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2003,24(4):302-305. |
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| Authors: | Hou Tiezhou Li Zhidan Wang Qiang Li Xiangru Wang Hong Tao Hong |
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| Abstract: | Objective To investigate the effect of fluoride on human tooth-germ cell proliferation and expression of related regulators. Methods By using an organ culture model of human tooth-germ in vitro, the effects of low (10?mg·L -1) and high (25?mg·L -1) concentrations of fluoride on cell proliferation were observed histologically and the expression of cyclinD 1 and proliferating cell nuclear antigen (PCNA) were detected by immunohistochemistry staining and image analysis technique. Results Fluoride inhibited cell proliferation and evidently decreased the expression of cyclinD 1 and PCNA. Conclusion Fluoride may inhibit tooth-germ cell proliferation by inhibiting the expression of cyclinD 1 and PCNA, which leads to the abnormal development of teeth. |
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| Keywords: | fluoride tooth-germ organ culture cyclinD PCNA |
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